电针对脑缺血再灌注大鼠缺血脑区IL-1β和TNF-α mRNA表达的调节  

Effect of Electroacupuncture on the Expression of IL-1β mRNA and TNF-α mRNA of Cerebral Neurons in Cerebral Ischemia-reperfussion Rats

作　　者：程玲[1] 骆明军[1] 明建扩[2] 陈邦国[1] 

机构地区：[1]湖北中医学院,武汉430061 [2]华中科技大学同济医学院,武汉430061

出　　处：《针刺研究》2003年第3期174-177,共4页Acupuncture Research

基　　金：湖北省自然科学基金资助 (批号 2 0 0 0J0 4 1)

摘　　要：目的 :探讨早期针刺治疗抗脑缺血再灌注炎性损伤的机制。方法 :采用大脑中动脉线栓法制备MCAO缺血再灌注模型 ,应用原位杂交的方法观察脑缺血再灌注及电针对大鼠大脑皮质、纹状体IL 1 βmRNA、TNF αmRNA表达的影响。结果 :脑缺血再灌注后 ,缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达增高 (模型组与正常组、假手术组比较P <0 0 1 ) ;电针可显著降低缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达 (电针组与模型组比较P <0 0 1 )。结论 :早期针刺治疗可能通过下调缺血脑区IL 1 βmRNA、TNFObjective: To explore into mechanisms of early treatment of cerebral ischemia-reperfusion (IR) by acupuncture in resisting inflammation injury in rats. Methods: Forty Wistar rats were randomly and evenly assigned to control, sham-operation, IR-6hr, IR-12hr, IR-24hr, IR-6hr-EA, IR-12hr-EA and IR-24hr-EA groups. Cerebral IR model was established by occlusion of the middle cerebral artery for 30 min and then removal of the occlusion for 6, 12 and 24 hr respectively. Electroacupuncture (EA, 4～16 Hz, 1～3 V, 30 min) was delivered via acupuncture needles inserted into 'Shuigou'(GV 26) and 'Baihui'(GV 20). The expression of IL-1β mRNA?TNF-α mRNA of neurons of the cerebral tissue was displayed using in situ hybridization technique after IR and assayed with a spectrometer system. Results: When compared with control group, the expression of IL-1β mRNA and TNF-α mRNA in the cerebral cortex and striate body on the ischemic side in IR-6hr, IR-12hr and IR-24hr groups increased significantly (P<0.01); while compared with IR-6hr, IR-12hr and IR-24hr groups separately, the expression of IL-1β mRNA and TNF-α mRNA of IR-6hr-EA, IR-12hr-EA and IR-24hr-EA groups was down-regulated considerably (P<0.01) despite being still higher than those of control group evidently. It suggested that EA could suppress the IR-induced over-expression of IL-1β mRNA and TNF-α mRNA of neurons in the brain tissues. Conclusion:The protective effect of acupuncture on cerebral neurons under acute cerebral infarction condition is possible through its downregulative action on IL-1β mRNA and TNF-α mRNA expression.

关 键 词：电针 脑缺血再灌注 脑区 IL-1Β TNF-Α mRNA 表达 调节 损伤机制 

分 类 号：R246.6[医药卫生—针灸推拿学] R394[医药卫生—中医临床基础]