针刺对肥胖大鼠神经肽Y及其基因表达的影响  

Effect of Acupuncture on Levels of Neuropeptide Y in the Blood and Hypothalamus and Its Gene Expression in Obese Rats

作　　者：刘志诚[1] 孙凤岷 赵东红[3] 张中成[1] 吴海涛[1] 孙志[1] 朱苗花[1] 魏群利[1] 李朝军[1] 

机构地区：[1]南京中医药大学,南京210029 [2]南京人口管理学院,南京210042 [3]南京师范大学,南京210000

出　　处：《针刺研究》2003年第3期192-198,共7页Acupuncture Research

基　　金：国家自然科学基金资助项目 (NO 39970 92 3)

摘　　要：目的 :探讨针刺减肥相关的细胞分子机制。方法 :采用RT PCR和NorthernBlot技术测定下丘脑神经肽Y(NPY)基因表达水平 ,放射免疫分析测定血浆和下丘脑NPY的含量 ,观察针刺治疗前后肥胖大鼠体重、Lee’s指数、体脂、血浆和下丘脑NPY的含量以及下丘脑NPY基因表达的变化。结果 :肥胖的大鼠体重、Lee’s指数、体脂、血浆和下丘脑NPY的含量以及下丘脑NPY基因表达均显著高于正常大鼠。针刺治疗取得良好减肥疗效的同时 ,肥胖大鼠血浆和下丘脑NPY含量以及下丘脑NPY基因表达水平明显回降。结论 :针刺对肥胖机体中枢和外周NPY水平的良性调整作用以及抑制下丘脑NPY基因过度表达可能是针刺减肥的细胞分子机制之一。Objective:To explore into the underlying cellular and molecular mechanisms of acupuncture in anti-obesity. Methods:Thirty-six SD rats were evenly randomized into control, model and electroacupuncture (EA) groups. Obesity model was established by feeding the rats with high fat diet forage. 'Zusanli'(ST 36) and 'Neiting'(SI 34) were punctured and then stimulated electrically by an EA therapeutic apparatus (10 Hz, 1.5 V, 10 min), once daily, continuously for 14 days. The expression of neuropeptide Y (NPY) gene in the hypothalamus tissue was determined with RT-PCR and Northern Blot techniques, and the contents of plasma and hypothalamic NPY were measured with radioimmunoassay. Changes of body weight, Lee's index and weight of fat tissue around the pericardium, kidney and epididymis were also observed before and after acupuncture. Results: Compared with control group, the body weight, Lee's index and body fat in model group increased markedly (P< 0.05～0.01), and when compared with model group, these indexes of EA group lowered significantly (P<0.05), showing that EA could reduce body weight. Comparison among three groups displayed that NPY contents in the serum and hypothalamic tissue of the model group were significantly higher than those of control group (P<0.01); and those of EA group were considerably lower than those of model group (P<0.01). RT-PCR and Northern Blot analysis displayed that compared with control group, NYP mRNA expression of model group increased significantly (P<0.01), while after acupuncture treatment NYP mRNA expression was reduced significantly in comparison with that of model group (P<0.05～0.01). Conclusion: Acupuncture induced reduction of serum and hypothalamic NPY levels and suppression of NPY mRNA over-expression in the hypothalamus may be its important cellular and molecular mechanisms in resisting obesity.

关 键 词：针刺 肥胖 神经肽Y 基因表达 影响 下丘脑 

分 类 号：R246.1[医药卫生—针灸推拿学] R394[医药卫生—中医临床基础]