时间分辨荧光免疫分析法测定人血清α-FP  

ULTRASENSITIVE TIME-RESOLVED FLUOROIMMUNOASSAY FOR HUMAN SERUM α-FP

作　　者：李振甲[1] 杨梅芳[1] 陈泮藻[1] 徐永源[2] 张安胜[1] 陈素娟[1] 

机构地区：[1]解放军总医院临床医学基础研究所,北京100853 [2]中国原子能科学研究院

出　　处：《上海免疫学杂志》1992年第2期103-105,共3页Shanghai Journal of Immunology

摘　　要：本文报告应用时间分辨荧光免疫分析技术测定人血清α-FP浓度。研究结果表明,将BAS引入该技术,使方法学稳定性和灵敏度有明显提高。本法批内CV为3.8～5.2％。批间CV为4.80～12.3％,最小检出值<1ng/ml,平均回收率为102.8％。健康成人测定参考值为5.56±5.38ng/ml(±SD)。聚苯乙烯微量滴定孔条经戊二醛处理后可提高包被抗体含量。The article reports a method of ultrasensitive time-resolved fluoroimmunoassay (TRFIA)for AFP in human serum. The results showed that the introduction of Biotin-Avidin System into TEFIA would greatly improve the stability and sensitivity of the method. The intra and inter C.V values were 3.8-5.2% and 4.8-12.3% respectively. The lowest detection limit was less than 1ng/ml. The average recovery rate was 102.3%. The normal value for healthy subjects was 5.56±5.38ng/ml (x±SD). Pretre-atment of polystyrene microtiter strips with glutaraldehyde solution could, increase the amount of antibody coated onto the wells.

关 键 词：荧光免疫分析 α-FP 时间分辨 

分 类 号：R446.61[医药卫生—诊断学]