DNA末端原位标记技术研究慢性再生障碍性贫血骨髓CD_(34)^+细胞凋亡  

Study on Apoptosis of Bone Marrow CD_(34)^+ Cells in Chronic Aplastic Anemia Patients by DNA in Situ End Labelling

作　　者：魏学礼[1] 刘捷[1] 王树庆[1] 马传香[1] 纪恩美[1] 张圣明[2] 

机构地区：[1]潍坊医学院附属医院,山东潍坊261031 [2]潍坊医学院组织胚胎学教研室

出　　处：《潍坊医学院学报》2003年第1期7-9,共3页Acta Academiae Medicinae Weifang

基　　金：国家中医药管理局科研基金资助课题 (课题号 :0 0 - 0 1LP37);山东省科技厅科研基金资助课题 (课题号 :971 2 2 62 0 9)

摘　　要：目的　观察慢性再生障碍性贫血 (CAA)患者骨髓CD3 4+ 细胞凋亡状况 ,探讨CAA可能的发病机制。方法　采用单克隆抗体 -免疫磁珠分离系统 (MACS)分离纯化 39例CAA患者的骨髓CD3 4+ 细胞 ,用DNA末端原位标 (TUNEL)技术分析骨髓CD3 4+ 细胞原位凋亡状况。结果　CAA患者骨髓细胞的凋亡率为 (39.2 4± 12 .70 ) % ,健康对照组为 (8.5 0± 2 .30 ) % ,组间比较差异有显著性 (P <0 .0 1)。凋亡细胞可见核染色质皱缩、凝聚 ,但未见到核碎裂现象及典型的凋亡小体。结论　CAA患者骨髓CD3 4+ 细胞的过度凋亡是其质或Objective To observe apoptosis of bone marrow CD 34 + cells in chronic aplastic anemia (CAA) patients and to investigate the pathogenesis of CAA.Methods Bone marrow CD 34 + cells were separated from 39 CAA patients with immunomagnetic beads(MACS)and analysed with TUNEL labelling.Results Apoptosis rate of CD 34 + bone marrow cells in CAA patients was (39.24.±12.70)%,while in the control group (8.50±2.30) so that there was a significant difference between two groups (P<0.01).Chromatin condensation was observed in apoptosis cells while neither nuclear crashing nor apoptosis body was found.Conclusion Over apoptosis in CD 34 + bone marrow cells of CAA is one of the reasons why the quality or number of CD 34 + cells declines

关 键 词：DNA末端原位标记技术 贫血 再生障碍性 慢性 细胞凋亡 

分 类 号：R556.5[医药卫生—血液循环系统疾病]