饮水氯化消毒副产物——3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮的DNA损伤作用研究  

Study on DNA Damage Induced by Chlorinated By-Product MX in Drinking Water

作　　者：吴建军[1] 刘爱林[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,湖北武汉430030

出　　处：《环境与健康杂志》2003年第6期326-328,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30170794)

摘　　要：目的研究饮水氯化消毒副产物———3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮(MX)的DNA损伤作用。方法以人肝肿瘤细胞株HepG2为靶细胞,MX设10、30、100、300μmol/L(终浓度)4个剂量,以二甲基亚砜为溶剂对照,H2O2为阳性对照,应用单细胞凝胶电泳试验(彗星试验)研究MX对HepG2的DNA损伤作用。结果MX在30、100、300μmol/L3个剂量均可引起HepG2DNA链断裂,DNA迁移长度随MX浓度的增加而增加,且与溶剂对照相比差异均有显著性。结论饮水氯化消毒副产物———MX对HepG2有明显的DNA损伤作用。Objective To study DNA damage induced by chlorinated drinking water by-product MX.Methods Hu-man hepatocellular carcinoma cell line HepG2cells was selected as target cells to test the DNA damage induced by MX using single cell gel electrophoresis assay.In this study,the cells were treated with MX at final concentrations of10,30,100and300μmol/L.DMSO and hydrogen peroxide were administered as solvent control and positive control respectively.Results MX induced DNA single strand breaks at the concentrations of30,100and300μmol/L.The length of DNA migration induced by MX increased significantly in comparison with the solvent control(DMSO)in a dose-response manner.Conclusions Chlorinated by-product MX in drinking water could induce obvious DNA damage to human hepatocellular carcinoma cell line HepG2cells.

关 键 词：水 消毒 DNA损伤 彗星试验 

分 类 号：R994.6[医药卫生—毒理学]