骨髓移植造血重建过程中干/祖细胞增殖特性的研究<英文>  

Proliferative Characteristics of Hematopoietic Stem/Progenitor Cells in Hematopoietic Reconstitution after Bone Marrow Transplantation

作　　者：丁顺利[1] 褚建新[1] 赵钧铭[1] 姜露[1] 胡小莉[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》1999年第2期138-141,共4页Journal of Experimental Hematology

摘　　要：为了探讨骨髓移植造血重建过程中干/祖细胞增殖的特性,我们采用病理形态学、BMNC计数、抗5-FU BMNC计数、外源性CFU-S(12)和骨髓连续移植等方法,对造血重建过程不同阶段的干/祖细胞增殖特性,进行了动态的对比研究。实验结果表明,在移植后第3天,骨髓内可见少数淋巴样细胞呈小灶性分布,第5—9天细胞灶迅速扩大,细胞形态表现为淋巴样细胞,至第11天才出现不同阶段的分化细胞;第5—9天BMNC数和抗5-FU BMNC数迅速增加,随后增殖速度明显减缓;第9天骨髓的CFU-S(12)数和重建长期造血的能力高于第5天和21天的骨髓。本实验结果提示,骨髓移植后造血细胞呈小灶性分布,重建造血初期造血干/祖细胞迅速增殖而无明显分化表现,此阶段骨髓细胞的重建长期造血能力增强,提示造血干细胞有扩增。In order to explore the proliferative characteristics of hematopoietic stem/progenitor cells (HSC/HPC) during hematopoietic reconstitution after bone marrow transplantation (BMT), the bone marrow samples in various phases of engraftment were dynamically studied by means of histopathologic obeservation, BMNC count, 5-FU-resistant BMNC count, the number of exogenous CFU-S(12) and serial BMT. At the 3rd day after BMT there were a few of small foci of lymphocyte-like cells in the bone marrow. From the 5th day to the 9th day after BMT, they expanded rapidly, but no differentiation trend can be found until the 11th day. The number of BMNC and 5-FU resistant BMNC were greatly increased during the 5th and 9th day. Then, the expansion speed was remarkebly slowly. Comparing with the bone marrow at 5th and 21st day, the number of CFU-S(12) from bone marrow at 9th day was the highest, and its ability to reconstitute hematopoiesis in serial BMT was the best too. Hematopoietic cell distribution was a focal form in the early stage of reconstitution. At the beginning of bone marrow reconstitution there was a expansion phase of HSC/HPC without differentiation. In this phase, its ability to reconstitute hematopoiesis was the strongest. It was suggested that HSC may be amplified in special condition.

关 键 词：骨髓移植 造血干/祖细胞 细胞增殖 造血重建 

分 类 号：R457.7[医药卫生—治疗学] R331.2[医药卫生—临床医学]