应用RT-PCR分析真性红细胞增多症病人TCR Vβ亚家族T细胞的表达  

Analysis of the Expression of TCR Vβ Subfamily T Cells in Patients with Polycythemia Vera by RT-PCR

作　　者：杨力建[1] 李扬秋[1] 许敏华[2] 祁明芳[2] 郁志[2] 陈少华[1] 廖继东[1] 

机构地区：[1]暨南大学血液病研究室,广州510632 [2]广州暨南大学第一附属医院血液科,广州510632

出　　处：《中国实验血液学杂志》1999年第1期76-78,共3页Journal of Experimental Hematology

摘　　要：近几年,国外相继报道对T细胞受体(TCR)Vβ24个亚家族基因的分析,从而了解肿瘤或自身免疫性疾病病人的细胞免疫功能状态,并为临床上进一步开展特异性免疫治疗提供新的实验依据。本文利用RT-PCR方法分析3例真性红细胞增多症病人Vβ亚家族T细胞的表达情况。To investigate the expression of TCR Vβ subfamily T cells, 24 genes of TCR Vp subfamily were amplified in peripheral blood mononuclear cells from 3 cases with polycythemia vera using RT-PCR. The results showed that only 4 to 14 Vp subfamily T cells were found in samples from patients, the main expressed Vβ subfamily T cells are Vp 2, Vp 3, Vp 16 and Vp 21. The patients with polycythemia vera selectively expressed some TCR Vβ subfamily T cells, it may be a feature of the cellular immune disorder in polycythemia vera.

关 键 词：T细胞受体VΒ基因 T细胞 真性红细胞增多症 逆转录-聚合酶链反应 

分 类 号：R555.1[医药卫生—血液循环系统疾病]