Tx-TnT在不同发育时期的艾维茵鸡和洪山鸡肌肉组织中的表达分析  

Expression of the Skeletal Muscle Tx-TnT During the Different Developmental Stages Between Avian Broiler and Hongshan Chicken

作　　者：余梅[1] 蔡伟强[1] 金建平 张庆德[1] 曹建华[1] 李奎[1] 

机构地区：[1]华中农业大学动物科技学院动物分子生物学与育种实验室,武汉430070 [2]美国CaseWesternReserve大学医学院

出　　处：《Acta Genetica Sinica》2003年第12期1097-1100,共4页

基　　金：国家重点基础研究发展规划项目 (编号 :G2 0 0 0 0 1610 3 );湖北省自然科学基金 (编号 :99J10 2 )资助项目~~

摘　　要：以艾维茵鸡和湖北省地方鸡种洪山鸡为实验材料 ,借助特异性识别Tx残基肽的单克隆抗体 6B8,采用Western杂交方法 ,检测Tx TnT异构体在洪山鸡和艾维茵鸡 7个发育时期 (孵化第 14d、初生 1日龄、7、14、2 1、2 8和35日龄 )的胸肌和腿肌中的表达差异 ,并与胸肌重进行相关分析。结果表明 ,Tx TnT在腿肌和孵化第 14d的胸肌中均不表达 ,在初生 1日龄后胸肌中的表达随发育逐步增长 ,统计分析发现 ,Tx TnT在艾维茵鸡和洪山鸡胸肌中的表达量具有显著差异 (P <0 0 5 ) ,与胸肌重具有显著相关 (P <0 0 5 )。Tropnin T (TnT) is the troponin complex subunit that most strongly binds to tropomyosin (Tm).Various TnT isoforms through alternative mRNA splicing are specifically expressed during development and/or in different muscle types,indicating that these forms have specialized functions in muscle contraction.The Tx structure has been identified in TnT isoforms specifically expressed in the breast of Galliformes and Craciformes.In the present study,monoclonal antibody 6B8 that recognizes Tx epitope was used to detect the expression of Tx TnT isoforms during the different developmental stages of breast and leg muscles between Hongshan chicken and Avian broiler.The results confirmed that the Tx epitope is only present in the breast but not leg muscles of both chickens.The least square analysis showed the quantum of Tx TnT expressed in the breast muscles increased during development and had significant difference ( P <0 05) between Hongshan chicken and Avian broiler.The correlations between the expression quantum of Tx TnT and the breast muscle weight were 0 91236 for Hongshan chicken and 0 87624 for Avian broiler,respectively,and significant at 0 05 level.

关 键 词：鸡 Tx-TnT Western杂交 表达差异 

分 类 号：S831[农业科学—畜牧学]