DHPLC对猪肌肉组织差异表达EST的鉴定  

Identification of Differential Expression ESTs in Pig Muscle Tissue by DHPLC

作　　者：王翀[1] 陈瑶生[1] 李重生[1] 黄志宏[1] 田兴国[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《Acta Genetica Sinica》2003年第12期1085-1089,共5页

基　　金：国家重点基础研究规划"973"项目 (编号 :G2 0 0 0 0 1610 4);广东省自然科学基金 (编号 :0 10 2 77)项目资助~~

摘　　要：采用DHPLC系统和LabWork4 0图像分析软件 ,对大白猪和广东地方品种蓝塘猪眼肌组织差异显示EST进行鉴定 ,两种方法均证实了差异显示条带的真实性。结果表明 :用DHPLC系统能够准确而简捷的检测不同组织中mRNA表达的差异 ,是 1种鉴定基因表达差异的有效方法 ,在研究基因表达 。The differential expression ESTs in muscle tissue of Large Yorkshire pig and Lantang pig,one of the local pig breeds in Guangdong province,were identified with DHPLC system and graphic analysis software LabWork4 0,and both methods verified the true differential display bands.The results showed that DHPLC can quickly and accurately detect differential expression mRNA profiles of different tissues,and can be widely used to study mRNA expression,differential expression of different animal tissues under variant environments.

关 键 词：变性高效液相色谱(DHPLC) MRNA 差异表达 猪 

分 类 号：S828[农业科学—畜牧学]