用合成肽研究对丙型肝炎病毒抗原决定簇的抗体反应  

STUDIES ON ANTIBODY RESPONSE VERSUS HEPATITIS C VIRUS BY SYNTHETIC PEPTIDES

作　　者：闻玉梅[1] 何丽芳[1] 李雪萍[1] 屠红[1] 张旭红[1] 吴长龙[2] 

机构地区：[1]上海医科大学微生物学教研室 [2]卫生部上海生物制品研究所

出　　处：《上海医学》1992年第6期311-314,共4页Shanghai Medical Journal

摘　　要：选择丙型肝炎病毒的核衣壳(C)区及非结构蛋白(NS)区,分别合成了5段肽(EIA-1、EIA-2、EIA-3、EIA-4和EIA-5)。158份抗-HCV阳性血清中,105份(66.5％)有对合成肽的抗体。以对EIA-2及EIA-5抗体的阳性率较高,分别占阳性总数的89％和85％。62份抗-HCV阴性血清中,12份有合成肽抗体,其中6份血清转氨酶升高,提示为丙型肝炎感染者血清。对选择合成肽段及作为抗原决定簇研究和应用的前景进行了讨论。Five synthetic peptides (EIA-1, EIA-2, EIA-3, EIA-4 and EIA-5) were synthesized according to sequences selected from the viral capsid protein (C) and nonstructural protein regions. Out of 158 anti-HCV positive sera (screened by Abbott 2nd generation kits), 105 were positive for anti-peptide antibodies; among which, antibodies versus EIA-2 and EIA-5 positive rates were the highest, being 89% and 85% respectively. Ameng 62 anti-HCV negative sera, 12 were positive for anti-peptide antibodies and among them 6 sera showed elevated ALT. It is highly suggestive that these 6 sera were from HCV infected individuals. The principles for selection of peptide sequences for mimicking antigenic epitopes and their prospects for future use are discussed.

关 键 词：合成肽 抗体反应 丙肝病毒 

分 类 号：R512.63[医药卫生—内科学]