用反相高效液相色谱分析胆石症患者胆汁的胆汁酸  

ANALYSIS OF CONJUGATED BILE ACIDS IN THE BILE OF PATIENTS WITH GALLSTONE BY REVERSE HPLC

作　　者：童赛雄[1] 盂承伟 林守诚[1] 

机构地区：[1]上海医科大学附属中山医院外科

出　　处：《上海医学》1992年第9期513-516,共4页Shanghai Medical Journal

摘　　要：用反相高效液相色谱法定量分析胆石症患者胆汁中的8种结合胆汁酸。色谱柱是C_(18)(粒度5μm,φ3.9mm×15cm)。流动相甲醇:0.05mol/L磷酸二氢钾(65:35V/V),用磷酸缓冲液调节到pH4.5,紫外检测器波长设置在20nm。45例胆石症测定结果,胆固醇性结石(CS)胆汁甘鹅去氧胆酸(GCDCA)的含量明显降低,以致三羟胆汁酸/二羟胆汁酸(CA/CDCA)的比值增高(0.81:0.59),胆色素性结石(PS)胆汁胆酸(CA)的含量降低,以致CA/CDCA的比值降低(0.42:0.59)。结果表明,胆石症患者的胆汁胆汁酸类型发生改变。The concentrations of 8 conjugated bile acids in the bile were determined by reverse high performance liquid chromatography (HPLC) in 45 patients with cholelithiasis and 10 normal controls. Compared with controls, the concentration of GCDA in the bile with chelesterol stones was significantly lowered and the CA/CDCA ratio elevated. In the bile with pigment stones, CA was significantly reduced so that the CA/CDCA ratio was markedly reduced.

关 键 词：胆结石 胆汁 胆汁酸 高效液相色谱 

分 类 号：R657.42[医药卫生—外科学]