维生素C对糖尿病大鼠视网膜毛细血管超微结构的保护作用  

The Protective Function of Vitamin C on Retinal Microvascular Ultrastructure in Diabetic Rats

作　　者：刘学政[1] 黄波[2] 周红丽[2] 马腾[1] 

机构地区：[1]锦州医学院解剖学教研室 [2]锦州医学院附属第一医院,辽宁锦州121001

出　　处：《锦州医学院学报》2003年第4期1-4,共4页Journal of Jinzhou Medical College

基　　金：辽宁省自然科学基金资助项目 (编号 0 0 2 114 )

摘　　要：目的　研究维生素C对糖尿病大鼠视网膜毛细血管超微结构的影响。方法　将SD大鼠 6 0只分为 5组 ,正常对照组 (CON) ,糖尿病 3月组 (DM 3) ,糖尿病 6月组 (DM6 ) ,糖尿病加Vc3月组 (DM3+Vc) ,糖尿病加Vc6月组 (DM 6 +Vc)。大鼠按 6 0mg/kg尾静脉注射链脲佐菌素 (STZ)以制备大鼠糖尿病模型。常规饲养 ,到期后麻醉 ,灌流固定 ,制作视网膜超薄切片 ,透射电镜观察。结果　超薄切片观察随着糖尿病病程发展 ,视网膜毛细血管内皮细胞、周细胞染色质凝集 ,核碎裂 ,线粒体发生空泡变 ,基底膜增厚 ,毛细血管管腔狭窄甚至闭塞 ,应用Vc后形态改变明显减轻。结论　Vc能延缓糖尿病大鼠视网膜毛细血管的形态改变 。Objective To study the influence of vitamin C on retinal microvascular ultrastructure in diabetic rats.Methods Animal models were induced on healthy adult SD rats.The rats were chosen and divided into following groups:normal groups(CON),diabetic rats at 3 months(DM3),diabetic rats at 6 months(DM6),diabetic rats at 3 months supplied with Vc(DM3+Vc),diabetic rats at 6 months supplied with Vc(DM6+Vc).Diabetes was induced with a single dose of 60 mg/kg of streptozotocin(STZ).When time is up,the eye was irrigated and studied by electronic-microscopy.Results For the electronic-microscopy,with the development of diabetic mellitus.The endothelial cell and pericyte cell changed,the chromatin condensed,the capillary caliber become narrow,some even closed.The basement membrane become thick.While in the groups supplied with Vc,the changes were not so worse.Conclusions Vc can delay the retinal changes of diabetic rats.So Vc has certain protection on the retinal capillary of diabetic rats.

关 键 词：糖尿病 维生素C 大鼠 视网膜毛细血管 超微结构 保护作用 

分 类 号：R587.2[医药卫生—内分泌] R977.23[医药卫生—内科学]