反相高效液相色谱法测定洛美沙星中空栓中洛美沙星的含量  

Determination of the Content of Lomefloxacin in Hollow Type Suppository by RP-HPLC

作　　者：翟伟宇[1] 韩翠艳[1] 程国平[1] 

机构地区：[1]齐齐哈尔医学院附属第一医院,齐齐哈尔市161041

出　　处：《中国药房》2003年第10期621-622,共2页China Pharmacy

摘　　要：目的 :测定洛美沙星中空栓中洛美沙星的含量。方法 :采用反相高效液相色谱法 ,色谱柱 :C18(4 6mm×250mm ) ;流动相 :0 05mol/L柠檬酸 -乙晴 (82∶18) ,用三乙胺调 pH值至4 0 ;流速 :1 0ml/min ;检测波长 :287nm。结果 :洛美沙星回收率为99 8 % ,精密度为0 6 %。结论 :本方法快速、准确 。OBJECTIVE:To establish the method for determining the content of Lomefloxacin in hollow type suppository of Lomefloxacin hydrochloride.METHODS:RP-HPLC was used with Eclipse XDB-C 18 column(4.6mm×250mm)and the mobile phase consisting of citric acid(0.05mol/L)-acetonitrile(82∶18)the pH of which was adjusted to4.0with triethyˉlamine.The flow rate was1.0ml/min and the detection wavelength was287nm.RESULTS:The average recovery of Lomeˉfloxacin was99.8%and RSD was0.6%.CONCLUSION:The method is rapid and accurate and can be used for determination of the content of Lomefloxacin in hollow type suppository.

关 键 词：反相高效液相色谱法 洛关沙星 中空栓 含量 

分 类 号：R927.2[医药卫生—药学]