氯气染毒致大鼠肺水肿时内皮素-1和E-选择素的变化  

Changes of the plasma levels of E-selectin and endothelin-1 in lung edema rats caused by chlorine

作　　者：吴山[1] 蒋志学[2] 孙明[2] 盛延良[2] 周毅[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400000 [2]佳木斯大学,黑龙江佳木斯154000

出　　处：《中国工业医学杂志》2003年第5期268-269,273,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　本研究观察氯气染毒致大鼠肺水肿时血中E 选择素和内皮素 1的变化 ,分析氯气引发这些因子变化与肺水肿产生的关系。方法　采用固相双夹心酶联免疫吸附法和放免技术测定大鼠血中E 选择素和内皮素 1浓度及肺脏干湿重量。结果　氯气染毒后大鼠血中E 选择素和内皮素 1浓度明显升高 ,并导致肺含水量增加。结论　氯气可升高E 选择素和内皮素 1浓度 ,随着E 选择素和内皮素 1浓度的升高出现肺水肿并逐渐加重 ,伴随这些因子降低肺水肿逐步缓解 。Objective To investigate the changes of the plasma levels of endothelin 1 and E selectin in lung edema rats by chlorine exposure.Method The rats were exposed to chlorine gas for 10 minutes to prepare lung edema model.Then,periodically take blood and lung tissure samples for various detection,such as wet and dry weights of lung tissues,plasma levels of E selectin and endothelin 1 (with double antibody sandwich quantitative ELISA method and radioimmunoassay,respectively).Result Compared with those in normal control group,the plasma levels of E selectin and endothelin l in chlorine exposed rats were obviously increased with the increase of wet weights of lungs.Conclusion Chlorine exposure can increase the plasma levels of E selectin and endothelin 1 which might play an important role in the pathogenesis of lung edema.

关 键 词：氯气 大鼠 肺水肿 E-选择素 内皮素-1 治疗 

分 类 号：R595.1[医药卫生—内科学] R-33[医药卫生—临床医学]