改构酸性成纤维细胞生长因子对小鼠胸腺细胞凋亡的影响  

Effect of Modified Acidic Fibroblast Growth Factor on Thymocyte Apoptosis Induced by Dexamethasone in Mouse

作　　者：曹定国[1] 李校坤[1] 付小兵[2] 程飚[2] 方利君[2] 

机构地区：[1]暨南大学生物医药研发中心,广州510632 [2]解放军304医院全军创伤修复重点实验室,北京100037

出　　处：《中国生物工程杂志》2003年第9期80-84,共5页China Biotechnology

基　　金：国家重大基础研究规划 ( 973 )资助项目 (G19990 5 42 0 4);国家 863计划资助项目 ( 2 0 0 1AA2 15 0 3 0 );国家自然科学基金面上与重点资助项目 ( 3 0 170 966;3 0 2 3 0 3 70 )

摘　　要：目的 :探讨改构酸性成纤维细胞生长因子 (MaFGF)对地塞米松 (DEX)诱导小鼠胸腺细胞凋亡的保护作用。方法 :利用地塞米松诱导小鼠胸腺细胞凋亡的模型 ,将实验分为空白对照组、DEX处理组、aFGF +DEX组和MaFGF +DEX组 ,采用DNA琼脂糖凝胶电泳和流式细胞仪两种分析方法 ,测定MaFGF对小鼠胸腺细胞凋亡的影响。结果 :空白对照组凋亡率为 1 68% ,DEX处理后小鼠胸腺细胞出现明显的细胞凋亡 ,凋亡率为 1 9 6% ,aFGF和MaFGF处理后的细胞凋亡受到抑制 ,凋亡率分别下降到 1 5 95 %和 1 2 93% ,MaFGF效应强于aFGF ,且以剂量依赖方式发挥作用。结论 :MaFGF具有以剂量依赖方式的胸腺细胞保护作用。AimTo explore the effect of modified acidic fibroblast growth factor on thymocyte apoptosis induced by dexamethasone in murine.Methods The experimental groups include:(1)controls,(2)DEX only,(3)aFGF+DEX,(4)MaFGF+DEX.The apoptosis of thymocyte induced by dexamethasone was observed by DNA agarose gel electrophoresis.Flow cytometry was used to quantitate the percentage of apoptosis.Results Dexamethasone was shown to induce apoptosis in thymocyte cells.The percentage of apoptosic thymocyte cells induced by dexamethasone was obviously lower in those groups pretreated with 100ng/ml wild aFGF or MaFGF compared with groups treated with dexamethasone only,and the percentage of apoptosic thymocyte cells pretreated with MaFGF was much lower than pretreated with wild aFGF.It showed the increase of antagonistic effect of MaFGF on thymocyte apoptosis in 50,100,150,200ng/ml MaFGF pretreated groups.Conclusion Modified acidic fibroblast growth factor protects against dexamethasone-induced apoptosis in thymocyte in dose-dependent manner,which independ on its non-mitogenic activity. ;

关 键 词：酸性成纤维细胞生长因子 胸腺细胞 细胞凋亡 地塞米松 

分 类 号：Q255[生物学—细胞生物学] Q26