月季切花衰老相关基因的差异显示及其序列分析  

Differential Display and Sequence Analysis of Senescence-associated Genes in Cut Roses

作　　者：白双义[1] 刘青林[1] 欧阳青[2] 蔡文启[2] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094 [2]中国科学院微生物研究所,北京100080

出　　处：《农业生物技术学报》2003年第2期154-157,共4页Journal of Agricultural Biotechnology

摘　　要：通过对不同瓶插寿命切花月季(Rosa hybrida)品种的DDRT-PCR方法,从长寿命的Pavarotti花瓣中扩增出22条cDNA特异片段,共得到9个cDNA片段的克隆,分别命名为b21、b32(b33)、b43、b51(b52、b814)、b618(b101、b108、b813)、b624、b131、b134和b186.序列测定和同源性检索的结果表明,来自b6的2个克隆b618和b624的序列不同,其中一个片段b618为439bp,其氨基酸序列与拟南芥(Arabisopsisthaliana)、柑橘(Citrus sinensis)、萝卜(Raphanus sativus)、苹果(Malus sp.)、葡萄(Vitisvinifera)和胡颓子(Elaeagnusumbellata)等查尔硐异构酶(CHI)相应区域的同源性为71%～79%;另一个片段b624为440bp,其氨基酸序列与拟南芥、豌豆(Pisum sativum)、欧洲油菜(Brassica napus)等的磷酸乙酰胆碱胞苷转移酶(CPCT)相应区域的同源性高达86%～90%.RT-PCR验证表明这2条cDNA片段为阳性结果.CHI涉及类黄酮色素的生物合成途径,CPCT参与胞苷二磷酸胆碱的合成途径,二者均与衰老进程相关.Using DDRT-PCR, twenty-two cDNA specific fragments were amplified from petal total RNA of Rosa cv. Pavarotti with longer vase life. Nine of those cDNA specific fragments were cloned, which were b21 , b32(b33), b43 , b51(b52 , b814), b618(b101 , b108 , b813), b624 , b131 , b134 and b186 respectively. Sequences blasted in GenBank showed that b618 and b624 derived from recombination plasmid b6 were two different sequences. b618 , which had 439 bp and 137 deduced amino acid, was 71%~79% homologous to that of corresponding encoding regions of chalcone isomerase(CHI), such as Arabisopsis thaliana, Citrus sinensis, Raphanus sativus, Malus sp., Vitis vinifera and Elaeagnus umbellata . While b624 , which had 440 bp and 134 deduced amino acid,was 86%~90% identical to the cholinephosphate cytidylyltransferase (CPCT) with Arabidopsis thaliana, Pisum sativum and Brassica napus. RT-PCR showed b618 and b624 were positive results. CHI was an enzyme involved in flavonoid biosynthesis pathway, while CPCT is responsible to CDP-choline pathway, both were related to the senescence process.

关 键 词：月季 切花 衰老 基因 差异显示 序列分析 

分 类 号：S685.12[农业科学—观赏园艺]