Thidiazuron对草莓外植体再生不定芽的影响  

Effects of Thidiazuron (TDZ) on Inducing Adventitious Shoot of Strawberry In Vitro

作　　者：尹淑萍[1] 金万梅[2] 王萍[2] 孟凡红[2] 韩振海[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]北京市农林科学院林业果树研究所,北京100093

出　　处：《农业生物技术学报》2003年第4期379-382,共4页Journal of Agricultural Biotechnology

基　　金：北京市科技新星计划资助项目(953812300)

摘　　要：采用TDZ(thidiazuron,N-phenyl-N'-1,2,3-thiadiazol-5'-ylurea)与生长素的不同组合诱导离体草莓(FragariaananassaDuch.)新星2号、Honeoye、Tudla和Darselect的叶盘和叶柄再生不定芽,建立了草莓高效再生系统。实验结果表明:新星2号和Tudla在MS+TDZ2.0mg/L+IAA0.1mg/L上再生频率分别为94.2%和87.9%,每叶盘平均再生不定芽为1.9和1.7个;Honeoye在MS+TDZ2.0mg/L+2,4-D0.1mg/L上再生效果好(89.1%);Darselect在MS+TDZ8.0mg/L+IAA0.1mg/L上叶盘再生频率较高(79.6%);同一基因型草莓叶盘再生效果比叶柄好。The adventitious shoots of strawberry(Fragaria ananassa Duch.)were induced by different combinations of thidiazuron(TDZ)and IAAs.The results showed that the shoot inducing rates of Xinxing No.2 and Tudla were 94.2% and 87.9% respectively; Number of shoots per leaf disc were 1.9 and 1.7 respectively in MS+TDZ 2.0 mg/L+IAA 0.1 mg/L medium. The regeneration frequency from Honeoye reached the highest figure of 89.1% in MS+TDZ 2.0 mg/L+2, 4-D 0.1 mg/L, while Darselect obtained the highest level of 79.6% in MS+TDZ 8.0 mg/L+IAA 0.1 mg/L mediums. The regeneration from leaf disc was better than that from petiole in the same genotype.

关 键 词：THIDIAZURON 草莓 外植体 再生 不定芽 :品种选育 

分 类 号：S668.4[农业科学—果树学]