HPLC法测定红花五味子木脂素的含量  

Determination of lignans of Schisandra rubriflora by HPLC

作　　者：王彦涵[1] 高建平[1] 郁韵秋[1] 陈道峰[1] 

机构地区：[1]复旦大学药学院,上海200032

出　　处：《中草药》2003年第10期950-952,共3页Chinese Traditional and Herbal Drugs

基　　金：高校博士点基金项目 (2 0 0 0 2 65 19);国家自然科学基金项目 (3 0 2 715 86);高校优秀青年教师教学和科研奖励基金项目(1999-71)

摘　　要：目的　测定不同产地、不同部位红花五味子的五味子酯甲 ( schisantherin A)、去氧五味子素 (五味子甲素 ,deoxyschizandrin)、五味子乙素 ( schisandrin B)和五味子丙素 ( schisandrin C)含量。方法　 HPL C法 ,使用 Sphere-clone ODS柱 ;流动相 :水 ( A) ,甲醇 ( B) ,梯度洗脱 :0～ 4 m in,70 % B;4～ 5 4 m in,70 %～ 10 0 % B;流速为 0 .4 m L/min;柱温 :2 5℃ ;检测波长 :2 5 4 nm。结果　五味子酯甲、甲素、乙素、丙素在红花五味子中含量分别为 0 .0 2 6 %～0.0 8 3% ,0 .0 0 7%～ 0 .94 5 % ,0 .0 0 2 %～ 0 .12 1% ,0 .0 10 %～ 0 .0 38% ,其中五味子甲素普遍存在 ,且果实中含量高于茎藤。结论　去氧五味子素是红花五味子果实的主要活性成分。Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils , which were collected from different areas Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm×4 6 mm, 5 μm); mobil phase, A: H 2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora

关 键 词：红花五味子 木脂素 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]