水稻体细胞突变体HX-3在细胞水平上对白叶枯病的抗性  

Resistance to Bacterial Leaf Blight in Somaclonal Mutant HX-3 at Cel l Level

作　　者：高东迎[1] 黄雪清[1] 刘永锋[2] 孙立华[1] 陈志谊[2] 刘蔼民[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014 [2]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《中国水稻科学》2003年第4期311-314,共4页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目 ( 30 2 0 0 1 72 );江苏省创新人才资助项目 (BK2 0 0 2 4 1 9)

摘　　要：对水稻抗白叶枯病体细胞突变体HX 3及其感病供体明恢 63在细胞水平上与白叶枯病菌的互作进行了研究。结果表明 ,接种水稻白叶枯病菌浙 173后 ,明恢 63愈伤组织的超氧化物歧化酶 (SOD)和过氧化物酶 (POD)活性均急剧大幅升高 ,O2 　 产生速率升高 ,而愈伤组织生长量减小 ,蛋白质含量下降。HX 3愈伤组织接菌后SOD、POD活性和O2 　　 产生速率与未接菌愈伤组织相似 ,愈伤组织生长量减小不明显。说明HX 3与明恢 63的细胞水平抗性存在较大差别 ,HXThe interaction between rice host and its pathogen Xanthomonas oryzae pv. oryzae (Xoo) at cell level was studied using a resistant somaclonal mutant HX-3 and its donor Minghui 63. After inoculation of Xoo strain Zhe 173(Chinese pathotype Ⅳ), the activity of superoxide dismutase(SOD) and p eroxidase(POD) in the callus of Minghui 63 was increased dramatically, and the active oxygen(O 2  ) was produced at a higher rate; Meanwhile, the callus grew slowly with the r eduction of protein content. However, the activity of SOD and POD , the product ion rate of O 2  and the fresh weight in HX-3 callus varied little after inoculation. It coul d be proposed that there were great differences between the resistance of HX-3 and Mighui 63 at cell level. There was no difference in resistance to bacteri al leaf blight in HX-3 between the plant and the callus.

关 键 词：水稻 体细胞突变体 白叶枯病 抗性 愈伤组织 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]