BADH基因转化水稻的方法比较  

Research on the Methods for Transforming BADH Gene into Rice

作　　者：卢德赵 祝水金[2] 钱前[1] 王慧中[3] 颜美仙[1] 黄大年[1] 

机构地区：[1]中国水稻研究所农业部水稻生物学重点实验室,浙江杭州310006 [2]浙江大学农业与生物技术学院农学系,浙江杭州310029 [3]杭州师范学院生物科学系,浙江杭州310036

出　　处：《中国水稻科学》2003年第4期323-327,共5页Chinese Journal of Rice Science

基　　金：国家转基因专项资助项目 (JY 0 3 B 0 8);浙江省自然科学基金资助项目;中国水稻科学基金资助项目

摘　　要：应用基因枪法与农杆菌介导法将来源于山菠菜的BADH (betainealdehydedehydrogenase)基因导入水稻。从获得转基因植株数量、转化率、转化植株PCR阳性率、外源基因拷贝数及其表达活性、T0 代植株的结实率对两种转化方法进行了比较。结果表明 ,用农杆菌介导法转化BADH基因的转化效率和PCR阳性率比基因枪法更高 ,且用农杆菌介导法得到的转基因植株T0 的结实率等农艺性状也优于基因枪法。The transformation effects of BADH gene (encod ing betaine aldehyde dehydrogenase) into rice by particle bombarment and Agr obacterium tumefaciens mediated were compared in transgenic plant number, t ransformation efficiency, rate of PCR positive plantlets, foreign gene copies and their expression activity, and seed setting rate of T 0 generation. Resu lts showed that transformation efficiency and rate of PCR positive plantlets by Agrobacterium tumefaciens mediated were higher than those by particle bombardment method. Moreover, the performance in seed setting rate of T 0 ge neration and some other agronomic traits of transgenic rice obtained from A grobacterium tumefaciens mediated gene transfer system were better compared w ith particle bombarment methodology.

关 键 词：BADH基因 转化 水稻 基因枪法 农杆菌介导法 

分 类 号：S511.03[农业科学—作物学]