烫伤大鼠肠粘膜下调蛋白质组的分离及鉴定  

Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats.

作　　者：王晓军[1] 孙永华 丁勤学[3] 冯喆[1] 曹原[1] 

机构地区：[1]北京市第六医院外科,100007 [2]积水潭医院烧伤整形科 [3]北京师范大生命科学院细胞生物学研究所

出　　处：《中华烧伤杂志》2003年第5期275-278,共4页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目 (G19990 5 42 0 )

摘　　要：目的　对烫伤后大鼠肠粘膜下调蛋白质组进行分离鉴定 ,探讨其在严重烫伤后肠粘膜损害发生中的作用。　方法　采用大鼠Ⅲ度烫伤模型 ,利用高分辨双向电泳 (2 DE)对肠粘膜组织进行蛋白质分离 ,用ImageMaster 2DElite图像分析软件对差异蛋白质中的下调蛋白质组进行鉴定。　结果　烧伤后 6、12h两组明确下调的蛋白质点数为 34,进行鉴定及分析的蛋白质 2 2个 ;与线粒体有关的下调蛋白质有线粒体乌头酸酶、丙酰辅酶A羧化酶、肝脏F1ATP酶重链A、肌钙蛋白 1、短链羟酰基辅酶A脱氢酶、P 电子转移 [传递 ]黄素蛋白α亚基等 6种 ;参与代谢的下调蛋白质有磷酸丙糖异构酶 1和细胞溶质环氧化物水解酶 ;与细胞骨架蛋白及基质蛋白有关的下调蛋白质有纤维单元素、类动力蛋白 5、肌钙蛋白 2和碱性肌浆球蛋白轻链 3共 4种 ;参与调控的下调蛋白质有糖皮质激素诱导蛋白、核因子 1 B2、BRCA1、雌二醇安息香酸盐转录因子、G 蛋白 β 2亚基、N 甲基 D 天门冬氨酸受体 1等 6种 ;与免疫调控有关的下调蛋白质有T细胞受体 V δ6和Ig重链V区蛋白 1。　结论　在大鼠烫伤后肠粘膜表达下调蛋白质中 ,以与线粒体有关及参与激素和细胞因子调控的蛋白质数量居多 ,说明大鼠烫伤后早期损伤发生机制与线粒体功能改变以及激素、细胞因子表达?Objective To explore the pathogenesis of postburn intestinal mucosal injury in scalded rats. Methods The rats inflicted with full thickness burn were employed as the model. The two-dimensional electrophoresis (2-DE) was employed to identify the down-regulating proteins from the differential proteins in scalded rats. Spot detection and matching were performed with Image Master 2D Elite. Mass spectrometry was performed on Bruker BIFLEX Ⅲ TOF. Results There are 34 points of proteins in intestinal mucosa in scalded rats which were down-regulated at 6 and 12 postburn hours. Among them 22 proteins were employed for the identification and analysis. Mitochodrial aconitase, alpha-propionyl -CoA carboxylase?heavy chain A of F1-ATPase in rat liver, Troponin short-chain of hydroxylacyl-coenzyme A dehydrogenase, alpha-subunit of P-electronic transferring flavoprotein(ETF) were down-regulating proteins correlated with mitochondria in intestinal mucosa in severely scalded rats. Triosephosphate isomerase 1 and cytosolic epoxide hydrolase were down-regulating proteins participated in metabolism of scalded rats. Fibromodulin, dynein-like protein, Troponin-2 and myosin light chain 3 alkali(MLC) were down-regulating proteins correlated with cellular skeletal protein. Glucocorticoid-inducible protein, nuclear factor1-B2, BRCA1, transcriptive factor EB(estradiol benzoate), β2 subunit of G-protein, N-methyl-D-aspartate receptor1(NMDAR) were down-regulating proteins participated in postburn regulation of intestinal mucosa in scalded rats. T-cell receptor-V-delta 6 and Ig heavy chain V region protein 1 were down-regulating proteins correlated with the immunomodulation of intestinal mucosa in scalded rats. Conclusion The down-regulating proteins of intestinal mucosa in scalded rats exhibited close relationship with mitochondria.

关 键 词：烫伤 大鼠 肠粘膜 蛋白质组 分离 鉴定 

分 类 号：R644[医药卫生—外科学]