水飞蓟素对小鼠肝细胞微粒体及线粒体膜流动性的影响  

The Effects of Silymarin on Hepatic Microsomal and Mitochond rial Membrane Fluidity in Mice

作　　者：吴东方[1] 彭仁秀[2] 叶丽萍[3] 于萍[3] 

机构地区：[1]武汉大学人民医院药学部,湖北武汉430060 [2]武汉大学医学院药理学系,湖北武汉430071 [3]武汉大学药学院,湖北武汉430072

出　　处：《中国中药杂志》2003年第9期870-872,共3页China Journal of Chinese Materia Medica

摘　　要：目的 :观察水飞蓟素silymarin对小鼠肝微粒体及线粒体膜流动性的影响。 方法 :以ANS ,DPH为荧光探针标记肝微粒体及线粒体膜浅层及深层 ,测定肝微粒体及线粒体膜荧光强度 (F) ,荧光偏振度 (P)及平均微粘度 ( η)。 结果 :水飞蓟素能增高肝微粒体及线粒体膜浅层流动性 ,同时能降低肝微粒体及线粒体膜深层流动性。CCl4 亦可导致肝微粒体及线粒体膜浅层流动性增加及肝微粒体及线粒体膜深层流动性降低 ,而给予水飞蓟素14 0 ,2 80mg·kg- 1后 ,能使增高的肝微粒体及线粒体膜浅层流动性降低 ,亦能使降低的肝微粒体及线粒体膜深层流动性增高。结论Objective: To observe the effects of silym arin on hepatic microsomal and mitochondrial membrane fluidity in mice. Method: Live r microsomal and mitoc hondrial membranes were labled by ANS and DPH. Membrane fluorensent intensity ( F ), fluorensent polarization(P) and microviscosily(η) of liver microsome and mitoc hondria were determined. Result: Sil increased the externa l membrane fluidities of liver microsome and mitochondria, and decreased the internal membrane fluidit ies of liver microsome and mitochondria. Pretreatment with CCl 4, the external me mbrane fluidity of liver microsome and mitochondria were increased, and the inte rnal membrane fluidities of liver microsome and mitochondria were decreased. Aft er given sil 140,280 mg·kg -1 , the increased external membrane fluidities of live r microsome and mitochondria were lowered , and the decreased internal membrane fluidities of liver microsome and mitochondria were enchanced in a dose-depende n t manner. Conclusion: The protective effects of sil on liv er injury may be relat ed to the recovery of the membrane fluidities of liver microsome and mitochondri a.

关 键 词：水飞蓟素 小鼠 肝细胞微粒体 线粒体膜 流动性 影响 中药 

分 类 号：R285.5[医药卫生—中药学]