香蕉花叶心腐病检测的两步法及一步法RT-PCR比较研究  

Comparative Studies on Two-step and One-step RT-PCR for Detection of Banana Mosaic Disease

作　　者：周莉娟[1] 郑伟文[1] 

机构地区：[1]福建省农科院生物技术中心,福建福州350003

出　　处：《江西农业大学学报》2003年第5期776-779,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省科技厅资助项目(2000I011)

摘　　要：香蕉花叶心腐病由黄瓜花叶病毒(CMV)引起,它有一个三分体正单链RNA基因组。从不同模板制备方法、RT和PCR过程引物浓度的选择等方面,用两步法RT-PCR对香蕉花叶心腐病的检测技术进行了研究。为了简化检测程序,在两步法RT-PCR检测程序的基础上,进一步研究香蕉CMV一步法RT-PCR检测技术,并对两种方法的检测灵敏度进行了比较。结果发现,两种方法都能从相当于100ng的香蕉叶组织中扩增到病毒带,但一步法RT-PCR的灵敏度比两步法略有提高。Banana mosaic disease is caused by cucumber mosaic virus(CMV), which posses a tripartite, positive-sense, single-stranded RNA genome. Two-step RT-PCR assay has been developed for detection of CMV through comparison of different protocols in RT-PCR. In order to simplify the procedures of the RT-PCR, one-step protocol was developed in this study to investigate CMV in banana tissue by following the two-step RT-PCR protocol. The results showed that CMV within 100ng banana infected tissue could be detected by both of one-step and two-step RT-PCR, and susceptibility of the later was slightly higher than the former.

关 键 词：香蕉 花叶心腐病 检测 两步法 一步法 反转录聚合酶链式反应 

分 类 号：S668.1[农业科学—果树学]