汉滩病毒核蛋白羧基端多肽原核表达载体的构建及表达  

Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

作　　者：李光玉[1] 黄长形[1] 潘蕾[1] 牟丹蕾[1] 李新红[1] 张颖[1] 杨为松[1] 白雪帆[1] 

机构地区：[1]第四军医大学唐都医院感染病诊疗中心,陕西西安710038

出　　处：《中国病毒学》2003年第5期428-431,共4页Virologica Sinica

摘　　要：本文为建立分型检测方法,探讨了汉滩病毒(Hantaan virus,HTNV)核蛋白羧基端多肽的抗原性。首先,分别构建编码HTNV核蛋白及其羧基端多肽的原核表达载体pRSETA-S、pRSETA-S-C;然后,将其转化入表达菌BL21(DE3)pLyss诱导表达,采用SDS-PAGE、Western-blot进行鉴定。我们成功构建了pRSETA-S及pRSETA-S-C原核表达载体。SDS-PAGE显示目的蛋白大量表达,呈不溶状态,Western-blot显现目的蛋白具有良好的抗原性。为大量制备分型用核蛋白多肽抗原创造了条件。To study the antigenicity of prokaryotic expressed products of Hantavirus(HV) S partial gene(502-1326bp) and obtain data for serotyping antigen, the prokaryotic expression vectors pRSET A-S, pRSET A-S-C were constructed respectively. The recombinant expression plasmids were transformed into BL21(DE3)pLySs. The expression product were analyzed by SDS-PAGE and Western-blot. The results showed that the proteins were expressed at high level respectively and could be recognized by patients ' (infected by HTNV) sera distinctly and peculiarly. The truncated NP (aa155 to 429) could be considered as serotyping antigen.

关 键 词：汉滩病毒 核蛋白 羧基端 原核表达 表达载体 

分 类 号：Q782[生物学—分子生物学]