两株水生呼肠孤病毒部分特性的比较  

Partial Characteristic Comparison Between Two Aquareovirus Isolates

作　　者：方勤[1] 丁清泉[1] 汪亚平[2] 朱作言[2] 

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071 [2]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《中国病毒学》2003年第5期464-467,共4页Virologica Sinica

基　　金：国家自然科学基金(30170730);"淡水生态与生物技术国家重点实验室"开放课题(2002FB05)

摘　　要：水生呼肠孤病毒为感染水生动物的一类病原体,隶属于呼肠孤病毒科新建水生呼肠孤病毒属。草鱼呼肠孤病毒(Grass carp reovirus,GCRV)是引起中国南方淡水养殖草鱼暴发性出血病病原,鲅鱼呼肠孤病毒(Threadfin reovirus,TFV)是引起海水养殖鲅鱼病毒病病原。本研究将GCRV与新加坡TFV分离株进行了部分特性比较研究。结果表明,GCRV与TFV均能感染CIK细胞,但对其它鱼类细胞系的敏感性有所差异。此外,凝胶电泳与逆转录聚合酶链式扩增显示,GCRV与TFV核酸属不同的基因型。在多肽特性上,证实了GCRV的5条主要结构多肽具有与FTV及水生呼肠孤病毒相似的特性。Western blot检测显示,草鱼呼肠孤病毒与TFV结构蛋白拥有部分相同的抗原决定簇。Aquareoviruses, which belong to a newly identified genus of the family Reoviridae, are agents infecting all kind of aquatic animals. Grass carp reovirus (GCRV) was isolated from outbreak grass carp fingerling in South China freshwater region, and Threadfin reovirus (TFV) is a Singapore isolate from acute diseased marine threadfin fingerling. In this study, the partial characterization between GCRV and TFV were compared. It showed that two viruses can cause CPE in CIK cell line, but not all the same in the other compared fish cell lines. In addition, their RNA genome compositions are different by both electrophoresis and RT-PCR. It is interesting to point out that, based on serological related Western blot analysis, the antiserum against GCRV could cross detecting some of the proteins found in TFV, but not all of the proteins. It suggested they may share some similar epitopes.

关 键 词：水生呼肠孤病毒 草鱼呼肠孤病毒 鲅鱼呼肠孤病毒 特性 

分 类 号：S941[农业科学—水产养殖]