衰老相关新基因CSIG的cDNA克隆和功能  

cDNA Cloning and Function of a New Cellular Senescence-associated Gene CSIG

作　　者：郭淑贞[1] 张宗玉[1] 童坦君[1] 

机构地区：[1]北京大学医学部生物化学与分子生物学系,北京100083

出　　处：《中国生物化学与分子生物学报》2003年第5期612-617,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金重点项目资助(No.3 993 0 170 );教育部科研基金;国家重点基础研究发展规划项目(G2 0 0 0 0 5 70 0 1)资助~~

摘　　要：为了获得 2BS细胞衰老过程中表达下降的差异基因片段Y6 2的编码序列 ,以cDNA末端快速扩增法获得细胞衰老相关新基因CSIG(cellularsenescenceinhibitedgene ,细胞衰老抑制基因 )的cDNA全长 .CSIGcDNA长 196 1bp ,编码 4 90个氨基酸 ,在多种重要组织中都有不同程度的表达 ;蛋白产物位于细胞核内特定位点 ,可能在核仁中聚集 .细胞转染表明 :CSIG可抑制细胞衰老并延长细胞寿限 。A new cellular senescence inhibited gene (CSIG) was cloned based on a previously obtained fragment with potential of higher expression in young versus senescent 2BS cells by rapid amplification of cDNA ends. The full length of CSIG cDNA is 1961 bp and encodes 490 amino acids. The CSIG mRNA expresses in many important human tissues with different extent. Its protein product located in the specific sites of nuclear might be concentrated in nucleolus.By ectopic expression,it was found that CSIG could delay cellular senescence of 2BS and extend cell lifespan,suggesting that it might participate in ribosomal biosynthesis or act as a transcription co\|factor to regulate cellular senescence.

关 键 词：CDNA克隆 细胞衰老抑制基因 细胞衰老 细胞转染 

分 类 号：Q255[生物学—细胞生物学] Q78