小鼠脾细胞凋亡释放RNA与自身免疫病的相关性  

Relationship Between RNA Released from Mouse Apoptotic Murine Splenocytes and Autoimmune Disease

作　　者：赵文璞[1] 刘相国[1] 王炜[1] 张岩[1] 吕平[1] 张玲[1] 高晓明[1] 

机构地区：[1]北京大学医学院免疫学系,北京100083

出　　处：《中国生物化学与分子生物学报》2003年第5期662-666,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：北京市科委重点项目资助 (No.95 5 0 2 12 10 0);"973"资助项目(No.2 0 0 1CB5 0 0 0 7)~~

摘　　要：在经放射线照射诱导的凋亡小鼠脾细胞培养上清中 ,可以提取到大量RNA ,用流式细胞仪检测发现凋亡细胞内的RNA量与正常细胞比较有所下降 .甲基绿 派若宁Y染色小鼠脾脏 ,脾细胞间质呈派若宁Y染色阳性 ,提示小鼠脾细胞也可能释放RNA .将小鼠脾脏研磨后 ,发现脾脏上清中也存在大量的RNA .采用小鼠脾脏上清RNA与脾脏细胞悬液总RNA的比值作为指标来衡量细胞凋亡释放的RNA量 ,并比较了BALB c及BXSB小鼠的差异 ,发现该比值在 3周 (72 % )、3月(5 8 4 % )、6月 (4 5 % )龄BALB c小鼠中呈下降趋势 ,而BXSB小鼠一直保持较高水平 (75 %～83% ) ,该比值在 3月龄、6月龄显著高于BALB c小鼠 (P <0 0 5 ) .同时 ,采用单相酶扩散的方法检测小鼠脾脏上清中RNA酶的活性 .6月龄BXSB小鼠的RNA酶活性显著低于同龄BALB c小鼠 (P<0 0 5 ) ,而 3周及Nuclear RNA are extruded from the cytoplasm of rat apoptotic thymocytes. RNA was extracted from the culture supernatant of murine splenocytes following apoptosis induction. The RNA content of apoptotic cells was less than that of living cells as determined in FACS analysis. Methyl green pyronin Y staining showed that RNA existed in extracellular space of mouse spleen, indicating that RNA was also extruded from spleen cells in vivo . The ratio of supernatant RNA to total spleen RNA was used to estimate the quantity of RNA extruded by apoptotic cells. The ratio of supernalant RNA to total RNA in BALB/c mice decreased with aging (72% for 3 weeks, 58.4% for 3 months and 45% for 6 months), while that of the BXSB mice of different ages remained at 75%-83%. RNase activity in the spleen supernatant, as measured by single radial enzyme diffusion assays, of 6 month old BXSB mice was significantly lower than that of BALB/c mice ( P <0.05), although no significant difference was not observed between BALB/c and BXSB mice of younger age.

关 键 词：细胞凋亡 脾脏 小鼠 自身免疫病 

分 类 号：Q255[生物学—细胞生物学]