金属离子对培养日本对虾肝胰腺细胞的影响  

Effects of Metal Ions on Cultured Hepatopancreatic Cells of Penaeus japonicus

作　　者：王宏伟[1] 王安利[2] 王维娜[2] 刘瑞兰[3] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]华南师范大学生命科学学院,广州510631 [3]西南师范大学生命科学学院,重庆400715

出　　处：《动物学杂志》2003年第5期6-9,共4页Chinese Journal of Zoology

基　　金：河北省重大科技攻关计划项目 (No.85-93-2 9)

摘　　要：研究了培养基中分别加入Ca2 + 、Mg2 + 和Zn2 + 对培养日本对虾肝胰腺细胞的影响 ,以测定细胞的RNA DNA值作为评价指标。当Ca2 + 浓度为 1g L时 ,细胞生长最好 ;本实验中随Mg2 + 浓度的增加 ,培养的日本对虾肝胰腺细胞的RNA DNA值也升高 ;当Zn2 + 浓度为 80 μg L时 ,其RNA DNA值最高 ;培养基中混合加入Ca2 + 和Mg2 + 有助于细胞贴壁生长。The effects of metal ions on the cultured cells from hepatopancreas of Penaeus japonicus was probed through the RNA/DNA ratio of the cultured cells. The cells grew best when the addition of Ca 2+ was 1?g/L;the RNA/DNA ratio of cultured cells raised following the increasing of the addi-tion of Mg 2+;the RNA/DNA ratio of cultured cells reached the maximum when the addition of Zn 2+ was 80?μg/L. The attachment of cultured cells was enhanced with the adding of Ca 2+ and Mg 2+ at the same time.

关 键 词：日本对虾 肝胰腺 细胞培养 金属离子 

分 类 号：Q954.45[生物学—动物学]