大鼠前连合核神经元对渗透压和血管紧张素Ⅱ刺激的神经性反应——脑片电生理研究  

NEURONAL RESPONSES OF THE ANTERIOR COMMISSURAL NUCLEUS TO OSMOTIC STIMULATION AND ANGIOTENSIN Ⅱ IN BRAIN SLICE IN THE RAT

作　　者：韩中胜[1] 段晓勤[1] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所神经生物学研究室,西安710032

出　　处：《神经解剖学杂志》1992年第2期191-196,共6页Chinese Journal of Neuroanatomy

摘　　要：在大鼠下丘脑离体脑片上,用电生理细胞外记录法研究了前连合核神经元的放电频率及其波形。实验观察到的前连合核自发放电神经元有不规则或规则的连续性放电活动,少数神经元有短阵性放电或不放电。这些神经元中的绝大部分可因脑片浸浴液中给予血管紧张素Ⅱ而被兴奋(放电频率增加),约1/3—1/2神经元对高渗透压刺激呈抑制性反应(放电频率减少),也有少数神经元可因高渗透压刺激而兴奋或无反应。前连合核的这些电生理特性大致与视上核、室旁核相似,也有一些与视上核、室旁核不同之处。对记录后的脑片进行组织化学及免疫组织化学染色的结果表明,记录部位在前连合核内,该部位有许多催产素样免疫阳性社经元。上述研究结果提示:前连合核的大细胞神经分泌神经元在水平衡的调节中起重要作用。The firing rates and patterns of neuronal activity in the anterior commissural nucleus (ACN) were studied on in vitro hypothalamic slices using electrophysiological techniques. Most of the ACN neurons observed exhibited irregular or regular continuous firing, a few in short-bursting pattern or silent. Most of these neurons were activated in response to bathing application of angiotensin Ⅱ, and a substantial number of neurons showed inhibitory response and some excitatory response to perfusion of hypertonic medium. The electrophysiological behavior of the ACNwas basically similar to that of the PVN and SON, although in these latter nuclei excitatory response to hypertonic medium is more common. The recording sites were histologically proved to be located in the ACN area, which was immunostained with antiserum to oxytocin. The present results indicate that the OT-LI neurons in the ACN are involved in the regulation of water balance.

关 键 词：前连合核 血管紧张素Ⅱ 渗透压 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]