脉冲式电磁辐射对大鼠血脑屏障影响的量效关系  

The study of dose-response relationship of pulsed electromagnetic radiation on rat blood-brain-barrier

作　　者：王琦[1] 任东青[1] 邝芳[2] 孙敬民[2] 王晋[1] 庞轶兵[1] 郭鹞[1] 郭国祯[1] 

机构地区：[1]第四军医大学预防医学系放射医学教研室,陕西西安710033 [2]第四军医大学神经生物研究所,陕西西安710033

出　　处：《疾病控制杂志》2003年第5期401-404,共4页Chinese Journal of Disease Control and Prevention

基　　金：全军"十五"指令性课题 (0 1L0 70 )

摘　　要：目的　研究脉冲式电磁辐射 (EMR)对大鼠血脑屏障 (BBB)影响的量效关系。方法　采用伊文思蓝静脉注射 ,荧光显微镜下观察不同脉冲次数的 EMR辐照对大鼠 BBB开放的影响。结果　伊文思蓝在 EMR诱发的大鼠 BBB开放局部呈现荧光斑 ;随着 EMR脉冲次数 (0～ 2 0 0次 )的增加 ,荧光斑数量增加、面积增大 ;荧光斑在 EMR组全脑的分布 ,以皮质、丘脑、下丘脑、小脑、尾壳核和延髓较多。结论　不同脉冲次数 EMR诱发大鼠 BBB开放程度不同 ,随脉冲次数增加 ,血管通透性增强 ,2 0 0次时达开放高峰 ,皮质区 BBB开放最明显。Objective To investigate the dose-response relationship of electromagnetic radiation on rat blood-brain-barrier (BBB). Methods Evans blue (EB) was used on the rat by intravenous injection, then observed the effect of pulsed electromagnetic radiation (EMR) in different pulses through fluorescent microscope. Results EB could form fluorescent faculae in brain where the BBB permeability had changed; the number and area of fluorescent faculae increased along with pulse numbers (0-200 pulses). Fluorescent faculae in EMR group were distributed over the rat brain, especially in cerebral cortex, thalamus, hypothalamus, cerebellum, caudate putamen and medulla oblongata. Conclusions Pulsed EMR in different frequence could induce different quantitative change of BBB permeability in rat brain, at 200 pulses the change was the most obvious, cerebral BBB permeability changed obvious more than others.

关 键 词：血脑屏障 脉冲式电磁辐射 脉冲次数 动物实验 

分 类 号：R594.8[医药卫生—内科学]