日本血吸虫感染致肝纤维化家兔肝脏微循环病变观察  

Hepatic Microcirculatory Pathology of Hepatic Cirrhotic Rabbit Induced by Infection of Schistosoma Japonicum

作　　者：李涛[1] 杨镇[1] 任大宏[2] 阮幼冰[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,武汉430030 [2]华中科技大学同济医学院基础医学院病理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2003年第5期519-521,F003,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No A30 170 92 0 )

摘　　要：目的　研究日本血吸虫感染致肝纤维化家兔的肝脏微循环病变。方法　对 30只日本血吸虫感染致肝纤维化家兔和 10只正常家兔取肝组织行光镜和电镜观察肝脏微循环病变。结果　HE染色示汇管区可见虫卵沉积 ,虫卵周围炎性细胞浸润。Mallory三色染色见肝内门静脉、肝动脉管壁纤维结缔组织增多 ,中膜平滑肌增殖肥大。透射电镜下见肝窦内皮受损严重 ,细胞大多崩解、破裂 ,窦内可见炎性细胞浸润 ,毛细胆管扩张 ,肝细胞肝窦面的微绒毛减少或断裂 ,肝窦呈毛细血管化。Objective To investigate the hepatic microcirculatory pathology of hepatic cirrhotic rabbits induced by infection of Schistosoma japonicum . Methods Liver tissues from 30 hepatic cirrhotic rabbits infected by Schistosoma japonicum and 10 normal rabbits were removed and observed under light and electron microscopy for hepatic microcirculatory pathology. Results Hematoxylin and Eosin (HE) staining indicated schistosome egg aggradation in the hilum and inflammatory cell infiltration around the schistosome eggs. Mallory trichrome staining showed hyperplasia of portal vein and hepatic artery wall fiber connective tissue, and proliferation and hypertrophy of the smooth muscle of middle membrane. Under transmission electron microscopy, it was found there was serious damage of hepatic sinusoidal endothelium cells, collapse of most cells, infiltration of inflammatory cells in hepatic sinusoidal, expansion of small bile duct, decreased or injured microfloss, and capillarization of hepatic sinusoidal.Conclusion Hepatic microcirculatory disturbances is one of the mechanisms by which schistosomiasis liver fibrosis induced portal hypertension develops.

关 键 词：日本血吸虫感染 肝纤维化 家兔 肝脏 微循环病变 观察 

分 类 号：R532.21[医药卫生—内科学] R575.2[医药卫生—临床医学]