黄芪对糖尿病造影剂肾损害的保护作用及机理研究  

Renal Protection of Mongelian Milkvetch Root on Radiocontrast Acute Renal Failure in Diabetic Rats

作　　者：刘毅[1] 林凡[1] 郑尘飞[1] 黄朝兴[1] 袁谦[1] 王禄萍[1] 许菲菲[1] 徐玉兰[1] 

机构地区：[1]温州医学院第一附属医院肾内科,温州325000

出　　处：《中国中西医结合肾病杂志》2003年第10期565-567,共3页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：浙江省卫生厅基金资助项目 (No .2 0 0 0A0 3 1)

摘　　要：目的 :探讨黄芪对糖尿病 (DM)造影剂 (CM)肾损害的保护作用及其作用机理。方法 :建立单侧肾切除糖尿病肾病模型 ,比较静脉注入 76 %泛影葡胺 (10mg/kg)前2 4h黄芪灌胃和生理盐水灌胃的大鼠血肌酐、内生肌酐清除率和血浆ET - 1变化情况 ,以及肾脏ET - 1、ET受体A、B(ETR -A、ETR -B)表达情况。结果 :糖尿病大鼠在黄芪灌胃后禁水 2 4h,注射 76 %泛影葡胺 36h后 ,大鼠内生肌酐清除率下降幅度与生理盐水灌胃组相比有统计学意义 (P <0 .0 5 ) ,血浆ET - 1浓度无统计学意义。黄芪灌胃组与生理盐水灌胃组相比 ,肾小管间质ET - 1、ETR -A、ETR -B表达明显低。结论 :黄芪对糖尿病造影剂肾损害的发生有一定的预防作用 ,这种预防作用可能与抑制肾脏局部内皮素系统作用有关。Objective:To study the renal protection of mongelian milkvetch root on radiocontrast acute renal failure in diabetic rats.Methods:Streptotocin-induded diabetic rats were randomly assigned into 3 groups: diabetic rats(n=6), diabetic rats injected with diatrizoate(10 ml/kg,n=6) and diabetic rats treated with mongelian milkvetch root(1 g/250 mg,n=10)before diatrizoate injection(10 ml/kg). Renal function and plasma endothelin-1 was measured before and after injection. The protein expression of ET-1,ETR-A and ETR-B was determined by immuno-histochemistry.Results:In the diabetic rats treated with mongelian milkvetch root,reduction of glomerular filtration rate(GFR)was less than those without mongelian milkvetch root treatment before diatrizoate injection. There was no significant difference in plasma ET-1 concentration between two groups, but it was lower than that of normal rats. Immunochemistry analysis showed reduced expression of ET-1,ETR-A and ETR-B in the rats treated with mongelian milkvetch root.Conclusion:Mongelian milkvetch root has renal protection on acute renal failure in diabetic rats induced by contrast media, probably due to suppression of renal local endothelin system.

关 键 词：黄芪 糖尿病 造影剂 肾损害 肾保护 并发症 

分 类 号：R285.5[医药卫生—中药学]