核因子NF-κB在糖尿病大鼠肾组织中表达及洛丁新对NF-κB的影响  

Expression of NF-κB in Renal Tissue of Diabetic Rat and Effect of Lotensin on NF-κB

作　　者：李龙[1] 许韩师[2] 陈应强[1] 罗建华[1] 陈运芬[1] 方国强[1] 

机构地区：[1]贵阳医学院附属医院肾内科,贵阳550004 [2]中山大学附属第一医院风湿科,广州510089

出　　处：《中国中西医结合肾病杂志》2003年第10期568-570,共3页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：贵州省教委基金资助项目 (No .2 12 0 3 0 46);贵州省委组织部基金资助项目 (No .S2 0 0 1-8);贵州省省长基金资助项目 (No .F2 0 0 0 -5 )

摘　　要：目的 :了解糖尿病鼠肾组织NF -κB的表达和肾功能损害的关系 ,并观察洛丁新对NF -κB的影响。方法 :采用微波免疫组化染色法检测各组肾组织NF -κB表达水平 ,并分析其与尿蛋白及肾功能的关系。结果 :糖尿病鼠肾组织NF -κB表达较正常肾组织明显增高 ,与尿蛋白及血肌酐增高呈正相关关系。用洛丁新治疗后 ,肾组织NF -κB表达明显下降 ,并改善肾功能。结论 :NF -κB可能在糖尿病肾病发生中有重要作用。肾组织中NF -κB的表达可作为反应进行性肾损害的指标。Objective:To investigate the expression of NF-κB in renal tissue of diabetic rat, its relationship with renal impairment, and to investigate the effect of Lotensin on NF-κB in diabetic rat.Methods:A microwave-based immunohistochemistry was performed to detect the expression of NF-κB in renal tissue.Correlation between NF-κB expression and urine protein, renal function in diabetic rat was also examined. Results:The expression of NF-κB in diabetic rat was higher than that of normal control, number of positive cells expressing NF-κB in renal tissue of diabetic rat showed a strong correlation with renal function and urine protein. Lotensin significantly inhibited the expression of NF-κB and improved renal function partly.Conclusion:NF-κB may play an important role in the pathogenesis of diabetic rat and the number of positive expressing NF-κB cells may be considered as a predictor of progressive renal injury in diabetic rat.

关 键 词：核因子NF-ΚB 大鼠 肾组织 洛丁新 免疫组化 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]