凝血酶对大鼠血管平滑肌细胞增殖的影响  

The Role of Thrombin on VSMCs Proliferation

作　　者：王伶[1] 刘东[2] 蒋建红[2] 何明[3] 

机构地区：[1]江西医学院第二附属医院检验科,江西南昌330006 [2]江西医学院第二附属医院分子生物中心,江西南昌330006 [3]江西医学院药理教研室,江西南昌330006

出　　处：《江西医学院学报》2003年第5期37-40,共4页Acta Academiae Medicinae Jiangxi

基　　金：江西省自然科学基金资助项目 (0 0 40 0 38)

摘　　要：目的　探讨凝血酶对大鼠血管平滑肌细胞 (Vascularsmoothmusclecells ,VSMCs)的促增殖作用的量效和时效关系。方法　采用 4～ 10代的VSMCs的单细胞悬液用于实验。 0 .1,0 .5 ,1,3,10U/ml凝血酶刺激VSMCs 2 4h或 0 .5U/ml和 1U/ml凝血酶刺激VSMCs 0 ,1,6 ,12 ,2 4,48,72h ,采用MTS法和 β N 乙酰氨基己糖苷酶测定VSMCs细胞增殖率。结果　①不同浓度的凝血酶对VSMCs有明显促增殖作用 (P <0 .0 1) ;但凝血酶浓度为 0 .1～ 10U/ml在 2 4h时对VSMCs的促增殖作用无显著差异 (P >0 .0 5 ) ;② 0 .5 (U/ml)和 1.0 (U/ml)两种浓度的凝血酶促VSMCs增殖相对于对照组 ,在各时间段均有明显促增殖作用 (P <0 .0 5 )。增殖曲线呈双峰样改变 ,1h和 2 4h峰值说明凝血酶对VSMCs的促增殖作用不仅有迅速而短暂的作用 ,而且有后发迟缓的作用。结论　①凝血酶浓度在 0 .1～ 10U/ml之间促VSMCs增殖无浓度依赖性 ;②凝血酶促VSMCs的增殖在时间上呈双峰样改变。Objective To study the effect of thrombin on cultured Vascular Smooth Muscle Cells(VSMCs)proliferation.Methods Growth-arrested VSMCs were used in the experiment.Quiescent cells were exposed to control or thrombin(0.1,0.5,1,3,10 U/ml)and proliferation was quantified at 24 h.The cells were also exposed to control or thrombin(0.5 U/ml and 1.0U/ml)respectively and the cell proliferation was quantified at 0,1,6,12,24,48,72 h respectively.Results ①Thrombin-at various concentrations(0.1～10 U/ml) significantly induced VSMCs proliferation at 24 h(P<0.05),but the effects of various concentrations of thrombin(0.1～10 U/ml)were similar to each other (P>0.05).②Compared with control group,cell proliferation was significantly induced by thrombin(0.5 U/ml and 1U/ml)at selective time(1,6,12,24,48,72 h)(P<0.05),and two proliferation curves were similar(P>0.05).The proliferation curve had two peaks,one at 1 hour,and the other at 24 h.Conclusion ①thrombin stimulated proliferation of VSMCs in a dose-independent with concentration from 0.1 U/ml to 10U/ml.②The cell proliferation induced by thrombin has two peaks at 1 hr and 24 hr.

关 键 词：凝血酶 血管平滑肌细胞 细胞增殖 量效关系 时效关系 心血管疾病 

分 类 号：R541[医药卫生—心血管疾病]