遗传标记AFLP在西门塔尔牛、延边牛、南阳牛品种鉴别中的应用  

The Application of AFLP Markers in Breed Discrimination of Simmental, Yanbian, Nanyang Cattle

作　　者：杨兴元[1] 许尚忠[2] 于汝梁[2] 周忠孝[3] 

机构地区：[1]中国农业大学生物学院,北京100094 [2]中国农科院畜牧研究所,北京100094 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《黄牛杂志》2003年第5期8-11,19,共5页Journal of Yellow Cattle Science

基　　金：九五国家科技攻关项目(96-003-04-01)资助.

摘　　要：分子遗传标记AFLP是建立在PCR和RFLP技术上的一项新的DNA指纹技术,可广泛应用于种系鉴定、遗传多样性检测、群体分类研究、构建遗传图谱、标定目的基因等方面。其最适应范围是利用AFLP技术鉴定品种的质量和纯度,进行基因型的指纹鉴定。本文利用遗传标记AFLP,选用已发表的牛AFLP标记引物,通过PCR方法获得多态性丰富、重复性好的西门塔尔牛、延边牛、南阳牛等3个牛品种的AFLP的DNA指纹图谱,并对其进行比较。结果表现:3个牛品种都有其特殊的指纹图谱,而且有其特异的条带,西门塔尔牛(188kb)、延边牛(321kb、212kb)、南阳牛(556kb、384kb),从而为这3个牛品种的鉴别提供了依据。AFLP is a new DNA fingerprint technology, which is widely used in breed and line discrimination, genetic diversity detection, population classification, genetic map construction, target gene mapping, and genotype fingerprinting discrimination for qualify and purification breed control. Polymorphic and repeatable AFLP fingerprinting of Simmental, Yanbian, Nanyang Cattle were made with published AFLP primers, results showed that every breed had specific fingerprinting composed of specific bands, 188 kb, 321 kb and 212 kb, 566 kb and 384 kb for Simmental, Yanbian, Nanyang Cattle, respectively.

关 键 词：遗传标记AFLP 西门塔尔牛 延边牛 南阳牛 品种鉴别 DNA指纹技术 指纹图谱 

分 类 号：S823.2[农业科学—畜牧学]