中国2002年脊髓灰质炎实验室网络的运转与监测  

The Surveillance and Running Status of Poliomyelitis Laboratory Network in 2002 in China

作　　者：李杰[1] 祝双利[1] 张勇[1] 赵蓉[1] 侯晓辉[1] 王东艳[1] 陈立[1] 张礼璧[1] 温宁[2] 徐涛 许文波[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]中国疾病预防控制中心免疫规划中心,北京100050 [3]中国疾病预防控制中心信息中心,北京100050

出　　处：《中国计划免疫》2003年第5期280-282,共3页Chinese Journal of Vaccines and Immunization

摘　　要：20 0 2年全国脊髓灰质炎 (脊灰 )实验室网络采集粪便标本的急性弛缓性麻痹 (AFP)病例 5 30 8例 ,分离到的所有毒株均做了血清定型 ,其中脊灰病毒 (PV)I型 5 5例 ,Ⅱ型 181例 ,Ⅲ型 97例 ,混合型 83例 ,脊灰与非脊灰肠道病毒 (NPEV)混合 2 7例 ,PV分离率为 8 3%。分离到NPEV4 5 8例 ,分离率为 8 6 %。 2 0 0 2年 ,31个省 (自治区、直辖市 ,下同 )疾病预防控制中心 (CDC)脊灰实验室均通过职能考核 ,成绩合格。对 11个省级脊灰实验室还进行了现场考核 ,全部通过考核认证。中国CDC病毒病预防控制所国家脊灰实验室对送检的 5 0 3株均做了聚合酶链反应 限制性酶切片段多态性分析 (PCR RFLP)法型内鉴定 ,其中 4 5 1例为从AFP病例中分离到的。经检测未发现脊灰野毒株 ,PV疫苗株I型 6 4例 ,Ⅱ型 170例 ,Ⅲ型 99例 ,混合型 82例 ,Ⅰ、Ⅱ、Ⅲ型变异株分别为 3、19、5例。按照世界卫生组织要求对所有毒株 (份 )进行了酶联免疫吸附试验 (ELISA)法型内鉴定 ,总计 6 0 9株 ,其中疫苗类似株(SL) 5 75株 ,占所有送检毒株的 94 4 % ;非疫苗类似株 (NSL) 3株 ,占 0 5 % ;双反应毒株 (DRV) 2 8株 ,占 4 6 %。并对 6 8例送检毒株做了VP1段的基因测序 ,发现疫苗衍生脊灰病毒 (VDPV) 2株 ,分别在四川省和安徽省分离到 。We analyzed the running status of poliomyelitis laboratory network in China in 2002 There were 5308 stool specimens collected from AFP cases by31 provincial laboratory staff,and all the viruses isolated were typed by the neutralization test.Of the viruses tested,55 were type I poliovirus,181 were type Ⅱ,97 were type Ⅲ,83 were mixed polioviruses,27 were mixture of poliovirus with other enterovirus,and 458 were non-polio enteroviruses (NPEV) 31 provincial polio laboratories except the Tibet polio laboratory (because they sent data late)had passed the proficiency test,even 11 laboratories got credit from experts autoptically 503 viral isolates sent to National Laboratory for Poliomyelitis were intratypically identified by PCR-RFLP test,among these,451 isolates were from AFP cases We found no wild-type poliovirus,but 64 type Ⅰ,170 type Ⅱ,99 type Ⅲ vaccine-like viruses,82 type-mixed viruses,and 3,19,5 polio vaccine mutant viruses of type Ⅰ,Ⅱ,Ⅲ respectively We also made ELISA test according to WHO’s requirements,among total 609 single type viruses,575 were Sabin-like viruses(SL),3 were non-Sabin-like viruses(NSL),28 were double-reaction viruses(DRV) After that,we selected 68 viruses to determine the VP1 gene sequene,2 vaccine-derived polioviruses (VDPV) were identified (from Sichuan and Anhui Provoinces each),but no residue paralysis in patients was found and no evidence showed that the VDPV spread among the communities

关 键 词：中国 2002年 脊髓灰质炎 实验室网络 监测 数据统计 急性弛缓性麻痹 

分 类 号：R512.4[医药卫生—内科学]