里氏木霉RutC-30β-甘露聚糖酶的制备与纯化方法的研究  

STUDIES ON METHODS FOR PRODUCTION AND PURIFICATION OF MANNANASE FROM TRICHODERMA REESEI

作　　者：王和平[1] 范文斌[1] 张七斤 王龙[1] 郭绥芝 

机构地区：[1]内蒙古农业大学生物工程学院,呼和浩特010018 [2]动物科学与医学学院 [3]内蒙古华药药业公司

出　　处：《内蒙古农业大学学报（自然科学版）》2003年第3期44-48,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(39870558)

摘　　要：里氏木霉以槐豆胶为基础培养基、以乳糖为诱导物,用7.5升液态发酵罐发酵产生β-甘露聚糖酶,经两次硫酸铵分级分离(饱和度为40％～60％)和两次聚丙烯酰胺凝胶制备电泳,结果得到高纯度均一的单体β-甘露聚糖酶,经SDS-PAGE测得酶分子量为53KD,比活力为100u/mg,这为β-甘露聚糖酶单克隆抗体的制备提供了高纯度的样品。the β-mannanase from trichoderma reesei was induced by gum locust bean and lactose through 7.5L fluid ferment, and was purified by twice (NH4)2SO4 precipitation(40%-60% concentration saturated) and twice PAGE purification, highly purified β-mannanase was obtained. The molecular weight of the enzyme is 53KD,The specific activity is 100u/mg.

关 键 词：里氏木霉 RutC^30β-甘露聚糖酶 制备技术 纯化方法 酶分子量 组织活动力 

分 类 号：Q814[生物学—生物工程]