针刺对肥胖大鼠褐色脂肪组织产热作用的影响  

Effect of acupuncture on the thermogenic action in brown adipose tissue of obese rats

作　　者：刘志诚[1] 孙凤岷 赵东红[3] 张中成[1] 孙志[1] 徐斌[1] 吴海涛[1] 徐炳国[1] 闫润虎[1] 李朝军[3] 

机构地区：[1]南京中医药大学第二临床医学院,江苏省南京市210029 [2]南京人口管理学院生殖医学教研室,江苏省南京市210042 [3]南京师范大学分子生物学研究所,江苏省南京市210097

出　　处：《中国临床康复》2003年第27期3665-3667,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39970923)"针刺减肥的细胞分子机制研究"~~

摘　　要：目的:探讨针刺对肥胖机体褐色脂肪组织(brownadiposetissue,BAT)解偶联蛋白1(uncouplingprotein1,UCP1)和肾上腺素能受体(β-adrenergicreceptor,β-AR)基因表达的作用。方法:采用反转录聚合酶链反应(RT-PCR)和RNA印迹试验(NorthernBlot)测定UCP1和β-AR的基因表达水平,观察针刺治疗前后肥胖大鼠(SD大鼠,n=18)体质量、Lee's指数、体脂,BATUCP1和β-AR的基因表达变化。结果:肥胖大鼠体质量、Lee's指数、体脂均显著高于正常大鼠(t=7.51,9.15,8.20;P<0.01),而BATUCP1和β-AR的基因表达水平明显低于正常大鼠(RT-PCR分析:t=-5.66,-4.66;NorthernBlot分析:t=-4.25,-4.925;P均<0.01)。相关分析显示,肥胖指标与BATUCP1和β-AR的基因表达水平呈负相关(RT-PCR或NorthernBlot分析P<0.05～0.01)。针刺治疗取得良好减肥疗效同时,肥胖大鼠BATUCP1和β-AR的基因表达水平明显回升(RT-PCR分析:t=2.20,6.72;NorthernBlot分析:t=2.33,2.35;P均<0.05)。结论:BATUCP1和β-AR的基因表达异常低下可能是产生肥胖的重要因素之一。针刺促进肥胖机体BATUCP1和β-AR的基因表达可能是针刺减肥的细胞分子机制之一。AIM:To explore the effects of acupuncture on the expression levels of uncoupli ng Protein1(UCP1) and β-Adrenergic receptor(β-AR)gene of brown adipose tis sue(BAT) in obese organism. METHODS:The expression levels of UCP1 and β-AR gene of BAT were determined w ith RT-polymerase chain reaction (RT-PCR) and Northern Blot techniques.The cha nges of body weight,Lee's index, body fat, and the expression levels of UCP1 and β-AR gene of BAT in obese rats(SD rats,n=18) were observed before and after a cupuncture. RESULTS:The body weight, Lee's index, body fat in obese rats were all markedly higher than those in normal rats(t= 7.51,9.15,8.20,P< 0.01), but the expression levels of UCP1 and β-AR gene of BAT in obese rats were all obviously lower th an those in the normal rats(RT-PCR Analysis:t=-5.66,-4.66; Northern Blot Anal ysis:t=-4.25,-4.925,P< 0.01). There were negative interrelation between the ob esity index and the expression of UCP1 gene and β-AR gene of BAT(RT-PCR or No rthern Blot analysis,P< 0.05-0.01). After acupuncture theatment the marked body weight lose effect was achieved while the expression levels of UCP1 and β-AR gene of BAT obviously increased in obese rats(RT-PCR analysis:t=2.20,6.72, Nort hern Blot analysis:(t=2.33,2.35,P< 0.05).

关 键 词：针刺 肥胖 褐色脂肪组织 解偶联蛋白1 肾上腺素能受体 基因表达 

分 类 号：R259.892[医药卫生—中西医结合] R246.1[医药卫生—中医内科学]