低强度氦氖激光对结核性胸液中淋巴细胞凋亡的影响  

Low intensity He-Ne laser irradiation for apoptosis of lymphocyte in tuberculous effusion

作　　者：周向东[1] 张婷[1] 兰箭[1] 

机构地区：[1]重庆医科大学第二附属医院呼吸内科,重庆400010

出　　处：《中国医学物理学杂志》2003年第4期235-236,共2页Chinese Journal of Medical Physics

摘　　要：目的:探索氦氖激光可否促进结核性胸液消退及其细胞学机制。方法:采用原位末标记DNA技术观察结核性胸膜炎患者使用氦氖激光胸膜腔内和血管内联合照射后胸液中淋巴细胞凋亡的时相动力学改变。结果:1日1次低能量穴3mw雪氦氖激光血管内和3日1次抽液时胸膜腔内照射60min可明显缩短胸液淋巴细胞凋亡时间,治疗3日平均半数凋亡时间降至81.2h,与对照组的98.3h有明显差异穴P<0.05雪,照射6日降至73.1h,其后维持于低水平直至胸水完全消退。结论:低强度氦氖激光胸膜腔和血管内联合照射可显著促进胸液淋巴细胞凋亡,可能是其加快胸水消退的重要机制之一。Purpose: To explore the cellular mechanisms of He-Ne laster irradiation for accelerating the resolution of tuberculous pleural effusion. Methods: In situ end-labeling technique of fragmental DNA was used to detect the apoptosis dynamic features of pleural effusive lymphocyte in patients with tuberculous pleuricy by intravascular and intrapleural He-Ne laser irradiation. Results: The apoptosis times of pleural effusive lymphocyte were shorted obviously by low-power He-Ne laser irradiation (Intraviscular: 3 MW-1 time/day- 60 min. Intrapleural: 3 MW- 1 time/3 days- 60 min).The average half-apoptosis time was reduced to 81.2 h by 3 day's irradiation-while the time of patients without irradiation was 98.3 h(p<0.05)-and the time was reduced to 73.1 h by 6 day's irradiation and maintain at low level until the thorough resolusion of pleural effusion. Conclusions:Intravascular in company with intrapleural low-power He-Ne laser irradiation could accelerate the apoptosis process of pleural effusive lymphocytes obviously and short the duration of pleural effusion.

关 键 词：氨氖激光 结核性胸膜炎 凋亡 淋巴细胞 

分 类 号：R529.3[医药卫生—内科学] R318.51[医药卫生—临床医学]