根癌农杆菌介导深黄被孢霉△^6-脂肪酸脱氢酶基因转化大豆及其转基因植株再生  

Introduction of △6-Fatty Acid Desaturase Gene from Mortieralla Isabellina into Soybeans by Agrobacterium Infection

作　　者：卜云萍[1] 王广科[1] 胡国武[1] 孙红妍[1] 任勇[1] 李航[1] 李明春[1] 邢来君[1] 

机构地区：[1]南开大学微生物系,天津300071

出　　处：《药物生物技术》2003年第5期273-277,共5页Pharmaceutical Biotechnology

基　　金：天津市自然科学基金重点项目 (项目编号 0 1 380 2 51 1 );高等学校骨干教师资助计划项目

摘　　要：采用农杆菌介导的大豆子叶节转化系统成功的将深黄被孢霉△6 脂肪酸脱氢酶基因导入大豆。从发芽 5～ 7d的大豆无菌苗切取子叶节外植体 ,经农杆菌浸染和共培养后 ,在含 50mg L卡那霉素的选择培养基上培养 2～ 4周后 ,从子叶节处诱导出抗性不定芽。将不定芽转移到伸长培养基上 ,4～ 6周后长至 2～ 3cm高的再生苗。再将再生苗切下转入生根培养基 ,2～ 6周生根。生根后的再生植株经逐步锻炼移入花盆中 ,部分移栽成活的T0 植株能正常开花结荚。从T0 植株上收获T1种子 ,按株系种植。T0 和T1代经PCR检测和DNA分子杂交分析 。The △ 6 fatty acid desaturase gene fron Mor ti tieralla isabellina was introduced into soybean successfully with Agrobacter ium cotylendon node transformation system. Cotylendon node explants were prep a red from 5～7 day old seedlings, infected and co cultivated with Agrobacteriu m tumefaciens. Kanamycin resistant (Km r ) adventitious buds emerged af ter 2～4 weeks on selective medium. The buds were transferred onto elongation me dium and regenerated shoots grew to 2～3 cm high after 4～6 weeks. The shoots we re cut off and transferred onto rooting medium. After about 2～6 weeks, the root ed plantlets were hardened off and transferred into pots. A part of the plants c ould bear flower and set seeds normally. PCR and Southern blot analysis from the T 0 and T 1 plants proved that foreign gene were transferred and i ntegrated into soybean genome.

关 键 词：大豆 △^6-脂肪酸脱氢酶基因 转基因植株 农杆菌介导法 

分 类 号：Q78[生物学—分子生物学] S565.1[农业科学—作物学]