复智散对抗Aβ25-35神经细胞毒性机制的研究  

Study of Fuzhisan against Aβ25-35 toxicity to nerve cells

作　　者：温世荣[1] 王德生[1] 张景艳[2] 盛树力[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院神经内科,黑龙江哈尔滨150001 [2]北京首都医科大学宣武医院北京脑老化重点实验室,北京100053

出　　处：《中风与神经疾病杂志》2003年第5期431-433,共3页Journal of Apoplexy and Nervous Diseases

基　　金：黑龙江省 95攻关课题 (Z99C2 0 -2 4)

摘　　要：目的　研究自制中药复智散对 Aβ2 5 - 35神经毒性作用的影响及相关机制。方法　测定 Aβ2 5 - 35及复智散孵育下 SH- SY 5 Y神经母细胞瘤株的 MTT值 ,利用 Western Blot法检测 CREB、Bcl- 2、Cyt C的表达。结果　 Aβ2 5 - 35致培养神经细胞存活率下降、CREB和 Bcl- 2表达下调、Cyt C表达增加 ,复智散促进神经细胞存活、上调CREB和 Bcl- 2、减少 Cyt C释放入胞浆。结论　复智散通过对 CREB、Bcl- 2和 Cyt C的影响对抗 Aβ2 5 - 35的神经细胞毒性效应。Objective To study the effect of Fuzhisan,a Chinese herb,on Aβ25-35 toxicity to nerve cells,and to investigate the possible mechanism. Methods Incubate neuronblastoma cells(SH-SY5Y) with Aβ25-35 and Fuzhisan. Detect exprission of CREB,Bcl-2 and CytC by Western Blot. Results Aβ25-35 decreased the survival rate of nerve cells,low-moduated expression of CREB and Bcl-2,and increased expression of CytC. Fuzhisan improved viability of nerve cells,modutated expression of CREB and Bcl-2,and decreased CytC into cytoplasma. Conclusion Fuzhisan inhibits toxicity of Aβ25-35 to nerve cells by affecting CREB,Bcl-2 and CytC.

关 键 词：复智散 AΒ25-35 神经细胞 毒性 神经母细胞瘤 

分 类 号：R273.94[医药卫生—中西医结合]