随机扩增DNA多态性技术在A组链球菌分型上的应用  

Typing of group A streptocococci by random amplified polymorphic DNA analysis

作　　者：苏健[1] 董太明[1] 龚守芳[1] 吴红穗[1] 陈志红[1] 陈剑光[1] 朱振宇[2] 

机构地区：[1]广东省心血管病研究所,广州市510100 [2]中山大学北校区生化教研室,广州市2510080

出　　处：《岭南心血管病杂志》2003年第5期336-339,共4页South China Journal of Cardiovascular Diseases

基　　金：广东省医学科学技术研究基金资助 (课题号 :A2 0 0 0 0 73 )

摘　　要：目的　了解A组乙型溶血性链球菌的基因分型状况。方法　采用随机扩增多态性DNA技术 (RandomamplifiedpolymorphicDNA ,RAPD) ,对 1988～ 1994年度 ,从广东城市和农村 ,1993～ 1994年度 ,从湖北、吉林 9～ 11岁学龄儿童分离的 179株GAS进行RAPD分析。结果　①同一T型的菌株有特征性条带 ,但有些亚型带型间差异较大 ;②不同T型间可有相同的带型 ;③分型率比T分型高 ,达到96 6 % ;④有主导RAPD型菌株流行的情况存在。结论　RAPD方法对GAS分型及流行病学中有一定的应用价值 ,可以区别同一血清型不同地区来源的GAS。Objectives To analyze the distribution of group A of streptococcal genotype. Methods Random amplified polymorphic DNA (RAPD) analysis was evaluated the genomic DNA of 179 strains of GAS. Results ①The same T type strains have characteristic bands, but some strains were more hete rogeneous; ②There were the same RAPD patterns between different T type. ③The discriminatory power of RAPD analysis was better than T typing, 96 6% GAS were typed by RAPD analysis; ④There were domain RAPD type in some T type strains. Conclusions RAPD analysis is useful in GAS typing and epidemiologicai investigations. It can discriminate the same serotype GAS from different region.

关 键 词：A组乙型溶血性链球菌 基因分型 随机扩增DNA多态性技术 PCR扩增 

分 类 号：R378.12[医药卫生—病原生物学]