牙鲆的淋巴细胞表面抗原  

Study on surface antigen of lymphocyte of Paralichthys oliveaceus

作　　者：乌日琴[1] 张培军[1] 徐芃[1] 李军[1] 徐永立[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学开放实验室

出　　处：《水产学报》2003年第5期486-490,共5页Journal of Fisheries of China

基　　金：国家"973"项目资助(G1999012003;G1999012006)

摘　　要：To observe the surface microstructure features by using scanning electron microscope,and study the surface antigen on lymphocytes of Paralichthys oliveaceus by form rosette with sheep red bloods cells and the cross-reactivity by using monoclonal antibody against human CD.Under scanning microscope ,there are two kinds of lymphocytes,type I lymphocytes is characterized with being full of bumpiness and holes, the surface of type II lymphocytes is rather smooth with tiny finger like tubercle. The rosette formation of lymphocytes with sheep red blood cells is obvious however the rosette forming percents of lymphocytes is rather low,lower than 2%. The cross reactivity of lymphocytes with monoclonal antibody against human CD showed that there are 5.25%+0.38% of NK cells in peripherial lymphocytes of Paralichthys oliveaceus,and the CD25^+ percentage of lymphocytes is 5.25%+0.38%.No cross-reactivity with T-cell antigen,B-cell antigen and other activity antigen is found on the lymphocytes surface of Paralichthys oliveaceus.To observe the surface microstructure features by using scanning electron microscope,and study the surface antigen on lymphocytes of Paralichthys oliveaceus by form rosette with sheep red bloods cells and the cross-reactivity by using monoclonal antibody against human CD.Under scanning microscope ,there are two kinds of lymphocytes,type I lymphocytes is characterized with being full of bumpiness and holes, the surface of type II lymphocytes is rather smooth with tiny finger like tubercle. The rosette formation of lymphocytes with sheep red blood cells is obvious however the rosette forming percents of lymphocytes is rather low,lower than 2%. The cross reactivity of lymphocytes with monoclonal antibody against human CD showed that there are 5.25%+0.38% of NK cells in peripherial lymphocytes of Paralichthys oliveaceus,and the CD25^+ percentage of lymphocytes is 5.25%+0.38%.No cross-reactivity with T-cell antigen,B-cell antigen and other activity antigen is found on the lymphocytes surface of Paralichthys oliveaceus.

关 键 词：淋巴细胞 表面抗原 绵羊红细胞受体 NK细胞 T细胞 牙鲆 

分 类 号：Q959.4[生物学—动物学] Q26