超短波对兔急性肺损伤肺内小动脉血管壁的保护作用  

The protective effect of ultrashort wave on pulmonary arteriole wall in rabbits with acute lung injury

作　　者：周淑华[1] 蒋小燕[1] 杨朝晖[1] 王倩[1] 黄庆华[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院康复医学科,武汉430022 [2]华中科技大学同济医学院微生物教研室

出　　处：《中华物理医学与康复杂志》2003年第10期581-583,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖北省卫生厅资助课题 (No .38)

摘　　要：目的　通过超短波对兔急性肺损伤肺部的治疗 ,观察肺内小动脉 (SmallArtery ,SA)血管壁的变化 ,了解超短波对急性肺损伤肺内SA血管壁是否具有保护作用 ,并初步探讨其作用机制。方法　健康大白兔 18只 ,随机分为A、B、C 3组 ,每组 6只。A组为正常对照组 ;B组将大肠杆菌内毒素静脉注射 3 0min后 ,应用微热量超短波治疗 ,每天 1次 ,共治疗 7d ;C组将大肠杆菌内毒素静脉注射后不做任何治疗 ,观察 7d。各组兔分别在规定时间静脉注入空气 5 0ml处死 ,样本用 40g/L多聚甲醛固定 ,NADPH d组织化学染色 ,光镜观察。结果　A组SA血管内膜光滑 ,NADPH d组织化学染色呈深紫色强阳性反应 ,内皮细胞及平滑肌细胞分布均匀。B组血管扩张 ,管壁NADPH d组织化学染色、内皮细胞及平滑肌细胞分布与A组无明显差别。C组SA血管内膜增生 ,内膜下结缔组织水肿 ,NADPH d组织化学染色呈深紫色强阳性反应 ,中层平滑肌水肿、增生 ,血管壁厚薄不均 ,管腔变窄。图像分析结果显示 :C组一氧化氮合酶 (NOS)阳性染色细胞数明显高于A组 ,差异有极显著性意义 (P <0 .0 1) ;而经超短波治疗后的B组NOS阳性染色细胞数与A组比较 ,差异无显著性意义 (P >0 .0 5 )。结论　超短波能够通过调节NOS的活性 。Objective To observe whether the ultrashort wave therapy has protective effects on the arteriole in acute lung injury(ALI) induced by endotoxin,and to study the underlying mechanisms. Methods Eighteen rabbits were used and divided into three groups randomly: group A, the rabbits were intravenously injected with 5ml of 0.9% saline and served as the control group.Group B, ALI were induced by intravenous injection of endotoxin (0.7mg/kg.body weight) in the rabbits,who were then treated with 30min of ultrashort wave diathermy once daily for one week after the injection.Group C, the rabbits were not given any treatment after the intravenous injection of endotoxin, and observd for one week. All groups of the rabbits were sacrificed by injection of 50ml air at the predetermined time point. The sections of the lung samples were stained by NADPH d after embedded in 40g/L polymethanal and observed under microscope. Results In group A the endothelium of pulmonary vessels were smooth, the vascular walls were deeply stained by NADPH d.The endothelial cells and smooth muscle cells distributed symmetrically.There were no differences between group B and the control group in staining, distribution of endothelial cells and smooth muscular cells. In group C, the pulmonary vascular endotheliums were proliferative, the connective tissues under endothelium were edematous and were deepestly stained by NADPH d.In the media layer the smooth muscle cells were edematous and proliferative and the layer thickness were not symmetrical.The vascular cavity become narrow.Image analysis showed that the number of cells positive for NOS staining were significantly increased in group C when compared with that in group A ( P < 0.01 ). However, there was significant differences between groups B and A in terms of the number of cells positive for NOS staining ( P >0.05). Conclusion Ultrashort wave can significantly reduce the lesion and proliferation of endothelium induced by endotoxin and regulate the activity of NOS and dilate the

关 键 词：超短波 急性肺损伤 肺内小动脉 血管壁 保护作用 平滑肌细胞 

分 类 号：R454.1[医药卫生—治疗学]