冷适应大鼠脑垂体、下丘脑、脾淋巴细胞和血浆β-内啡肽及其mRNA的变化  

CHANGES OF β-ENDORPHIN AND ITS MESSANGER RNA IN PITUITARY,HYPOTHALAMUS,LYMPHOCYTES AND BLOOD PLASMA DURING COLD ACCLIMATION OF RATS

作　　者：徐扬[1] 杨泽田 苏成芝[1] 

机构地区：[1]第四军医大学分子生物学研究室

出　　处：《生理学报》1992年第1期45-53,共9页Acta Physiologica Sinica

摘　　要：本文采用β-内啡肽(β-EP)mRNA打点杂交,反相高效液相色谱和放免测定研究了冷适应SD雄性大鼠垂体、下丘脑、淋巴细胞(LC)和血浆β-EP及其mRNA的变化,结果为:(1)冷暴一周时垂体β-EP mRNA明显增加,从而激活细胞免疫功能。(2)免疫中枢下丘脑和LC β-EP mRNA在冷适应建立(冷暴二周)时增加。(3)血浆β-EP从冷暴二周起持续增加,增强细胞免疫功能。虽然LC和血浆β-EP产物增加,但是垂体β-EP mRNA量从冷暴二周起恢复到对照组水平,很可能是通过LC-垂体-下丘脑轴,信息反馈到中枢神经系统造成的。Changes of β-endorphin (β-EP) and its mRNA in pituitary (P), hypothal-amus(HT), lymphocytes (LC) and blood plasma (BP) during cold acclimation ofSD male rats were studied by β-EP mRNA dot blot, RP-HPLC and β-EP radio-immunoassay (RIA). Experimental results showed: (1) After cold-exposure for1 week pituitary β-EP mRNA increased significantly with the appearence ofstimulated cellular immune function. (2) β-EP mRNA in hypothalamic immunecenter and peripheral LC increased when cold acclimation of animals was estab-lished for a cold exposure of 2 weeks (C2W). (3) From C2W onward, plasma β-EP also continued to increase, indicating an augmented state of cellular immunefunction. As LC and plasma β-EP product continued to show increase, pituitaryβ-EP mRNA content recovered to control level from C2W onward possibly due toa feedback mechanism through LC-P-HT axis.

关 键 词：冷适应 垂体 下丘脑 淋巴细胞 血浆 

分 类 号：R392.1[医药卫生—免疫学]