阿片肽类物质对大鼠黄体细胞孕酮生成的影响  

EFFECT OF OPIOID PEPTIDES ON PROGESTERONE PRODUCTION BY RAT LUTEAL CELLS IN VITRO

作　　者：童国遐[1] 赵白鸽[2] 王忠兴[2] 顾敦瑜[2] 罗履广 程治平[1] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150086 [2]上海市计划生育科学研究所,上海200032

出　　处：《生理学报》1992年第3期269-274,共6页Acta Physiologica Sinica

摘　　要：本文观察了外源性阿片肽对大鼠离体黄体细胞孕酮生成的影响,结果表明:β-内啡肽以剂量-反应依赖方式促进黄体细胞孕酮生成,有效浓度范围是10^(-8)-10^(-6) mol/L;强啡肽仅在浓度为10^(-6)mol/L时才显示刺激孕酮生成的作用;而甲硫-脑啡肽无明显作用。μ-阿片受体激动剂DAGO和乙基吗啡也能明显促进孕酮的生成。纳洛酮可完全阻断β-内啡肽,DAGO和乙基吗啡的作用。由于大鼠血液中β-内啡肽含量较低,而卵巢局部具有较高浓度的β-内啡肽。因此,我们认为,β-内啡肽可能在卵巢局部参与黄体细胞孕酮生成的调节,是卵巢内促黄体因子之一,这种作用可能是由μ-型阿片受体介导的。The effect of exogenous opioid peptides on progesterone production by incu-bated rat luteal cells was studied. β-endorphin (β-EP) stimulated proges-terone production in a dose-dependant manner (10^(-8)--10^(-6) mol/L); dynorphinexhibited a stimulatory effect only at 10^(-6) mol/L, while Met-enkephalin had nosubstantial effect at dose from 10^(-10)--10^(-6) mol/L. μ-opioid receptor agonistsDAGO and morphine also stimulated progesterone production. The stimulatoryactions of β-EP, DAGO and morphine were reversed completely by naloxone.On account of the fact that the β-EP level in rat plasma is lower than that in theovary, it seemed that β-EP may be an intra-ovarian luteotrophic factor and beinvolved in the regulation of progesterone production. This action of β-EP maybe mediated by μ-opioid subtype receptors.

关 键 词：阿片肽 孕酮 黄体细胞 大鼠 

分 类 号：R339.22[医药卫生—人体生理学]