应用高效毛细管电泳技术快速分离尿肌酐  

Rapid determination of creatinine in urine by high performance capillary zone electrophoresis

作　　者：吴建国[1] 周永列[1] 张学军[1] 

机构地区：[1]浙江省人民医院,浙江杭州310014

出　　处：《上海医学检验杂志》2003年第6期353-355,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的　建立 1种快速测定尿中肌酐浓度的毛细管电泳方法。方法　用pH2 .5、0 .1mmol/L磷酸作为电泳缓冲液 ,利用非涂层石英毛细管 48.5cm× 5 0 μm(id) ,采用外标法定量 ,检测波长 191nm。 结果　本法在34 .5～ 8840 μmol/L浓度范围内呈良好的线性 (r =0 .998) ,日内和日间变异系数分别为 3.5 %、6 .2 %,平均回收率 94.7%。与苦味酸法有良好的相关性 (Y =1.0 0 4X +0 .0 0 4,r =0 .998,n =45 )。结论　本法线性范围宽、简单、快速 ,可应用于临床样品的检测。Objective To establish a method for the determination of creatinine in urine by capillary zone electrophoresis. Methods 48.5 cm×50 μm uncoated capillary, phosphoric acid buffer (0.1 mmol/L, pH2.5) and UV-detector at 191 nm wavelength were used in this method. Results The calibration curve showed a good linearity for creatinine in the concentration range of 34.5-8 840 μmol/L (r=0.998). Within-day CV and between-day CV for assaying the urine samples of creatinine were 3.5% and 6.2% respectively. The average recovery was 94.7%. We also compared the method with the Jaffe kinetic methed, and the results showed a satisfactory correlation (r= 0.998,n=45). Conclusions The method is simple, rapid and with wide linearity range, so it is useful for clinical application.

关 键 词：高效毛细管电泳技术 快速分离 尿 肌酐 

分 类 号：R446.12[医药卫生—诊断学]