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作 者:Zhao Weihong Hong Wanshu Zhang Qiyong JiangXinxin Wu Dingxun 赵卫红;洪万树;张其永;姜欣欣;吴鼎勋(Department of Oceanography,Xiamen University,Xiamen 361005,Fujian,China)
机构地区:[1]Department of Oceanography,Xiamen University,Xiamen 361005,Fujian,China
出 处:《Marine Science Bulletin》2003年第2期87-95,共9页海洋通报(英文版)
基 金:This study is supported by the FJNSF(No.B0210003);by the NSFC(No.30170739)
摘 要:Maturation of the 4th phase oocytes of mudskipper is induced in vitro by sexpheromones,the extract of ovary,testis and seminal vesicle,oxytocini and deoxycorticosterone(DOC).Maturation rates of oocytes are related to pheromones and their dosages.The single use ofprostaglandin E_1(PGE_1)is not effective in the induction oi maturation,but the combination ofPGE_1 with HCG promotes oocyte maturation rates.Maturation is induced by injections of the extractof the ovary at dosages of 0.01 cm^3/a fish,seminal vesicle extract at dosages of 0.1 cm^3/a fish,testis extract at dosage of 0.1 cm^3/a fish,or PGE_1 at dosage of 10μg/fish,respectively.Amongthem,seminal vesicle extract is the most effective in the induction of maturation.The artificialnests,with the extract of the ovary,testis or seminal vesicle inside,attract more heterosexualmudskippers than homosexual mudskippers to enter.Especially,the extract of seminal vesicle is themost effective in attracting females.Female mudskippers could be induced to spawn by the extractsof ovary,testis and seminal vesicle placed inside both the ceramic and sponge nests.More spawnedeggs and higher fertilization rates are observed in the ceramic nests than in the sponge ones.
关 键 词:boleophthamus pectinirostris sex pheromones maturation and spawning
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