小牛皮提取的胶原-微载体用于贴壁细胞大规模培养  

Cultivating Anchorage-dependent Animal Cells with Collagen and Denatured Collagen Microcarrier

作　　者：陈因良[1] 李雨田[1] 张书元 宋瑶君[1] 叶南章[1] 华平[1] 袁滔[1] 丁健椿[1] 

机构地区：[1]华东化工学院生化工程研究所

出　　处：《生物工程学报》1992年第2期157-163,共7页Chinese Journal of Biotechnology

摘　　要：由于胶原蛋白具有与含牛血清培养基中纤粘素(Fibronectin)一类物质相结合的特点,因而胶原是一类来源广泛的可用作制备细胞培养介质的优良基质。经过各种形式的细胞培养试验,以变性胶原为材料合成得到的GT-2微载体可成功地用于Vero、CHO、Bowes以及鱼类细胞的贴壁培养。培养规模包括不同容积的静置培养、滚瓶培养以及1.5L和20L进口和国产生物反应器的大规模细胞培养。It was shown that collagen substrates enhence the growth as well as the differentiation of many cells in culture. Collagen is the major fibrous element of animal bodies and the pure collagen was extracted from the new born calf skin by using chemical and biochemical methods. The analyses of ion exchange chromatography and electrophoresis show that the main components of denatured collagen are α monomers and β dimers. Collagen and denatured collagen solution were coated on peterj dishes and after drying by air aseptically, they were irradiated by ultra violet ray to crosslink collagen. On these membranes various cells were cultured and the denatured collagen was proved as a very good matrix for culturing anchorage-dependent cells. A kind of denatured collagen (gelatin) MC, GT-2 was developed by crosslinking gelatin with glutaraldehyde in a suspension polymerization process. These microcarrjers were sucessfully used in different culture scales to culture various anchoragedebendent cells such as Vero, CHO and Bowes. These scales include Tflasks, spinner bottles, roller bottles and 1.5, 2.5, 5.0 and 20 liters bioreactors.

关 键 词：生物反应器 胶原 微载体 细胞培养 

分 类 号：Q78[生物学—分子生物学]