猪垂体中猪生长激素基因的克隆和部分序列分析  

Cloning and Partly Sequencing of the Porcine Growth Hormone(pGH)Gene from Pituitary Gland

作　　者：杨青 朱宝利[1] 周顺伍[1] 齐顺章[1] 

机构地区：[1]北京农业大学动物生化教研室

出　　处：《生物工程学报》1992年第4期318-323,共6页Chinese Journal of Biotechnology

基　　金：博士点基金

摘　　要：从猪垂体中提取出DNA,以EMBL3噬菌体作载体,构建了染色体基因文库。用全长的猪生长激素(pGH)cDNA作探针,经原位杂交,筛选出5个阳性克隆。提取其中一个阳性克隆的DNA,通过斑点杂交,酶解和Southorn印迹,表明它含有全长猪生长激素基因;将Sma Ⅰ酶解后Southorn印迹为阳性的两个片段进行了亚克隆,对其中含有猪生长激素基因Sma Ⅰ位点上游序列的片段进行了部分序列分析,并和已发表的相应序列进行了比较。The high molecular weight DNA was isolated from porcine pituitary gland,and the genomic library was constructed using λ EMBL3 as the cloning vector. Five positive clones were identified by in situ hybridization with the full length pGH cDNA.Dot-blot hybridization,restriction analysis and Southern hybridization showed that one of the positive clones contains the whole sequence of pGH gene.Tow positive Sma I fragments were subcloned, one of which contains the upstream sequence from the Sma I site of pGH gene and subsequently were partly sequenced.We also compared the sequence that we obtained with the reported corresponding sequence of pGH gene.

关 键 词：猪垂体 序列分析 生长激素基因 

分 类 号：S188[农业科学—农业基础科学]